摘要
目的:研究急性低氧暴露下运动模拟急性高山病(AMS)发生中血清ACE活性及AngⅡ水平的变化趋势及ACE、AT1R基因多态与其水平的关系。方法:模拟海拔4800 m低氧环境,49名北方汉族大学生急性暴露6 h,其中入舱30 min后以恒定负荷蹬车20 min,LLS量表评价AMS。常氧安静(NM)和急性低氧暴露结束(HY)时测定血清ACE活性及AngⅡ水平。PCR-RFLP法检测受试者ACE基因A-240T、A2350G位点及AT1R基因A1166C位点的基因型和等位基因频率。结果:1)低氧暴露后,AMS组的ACE活性轻微上升,非AMS组轻微下降,两组间的ACE活性变化量(△ACE(HY-NM))差异不显著。两组的AngⅡ水平均下降,但AMS组下降显著,非AMS组下降不显著,两组间的AngⅡ变化量(△AngⅡ(HY-NM))差异不显著。2)不同基因型和等位基因携带者的AMS发生率和AMS评分变化趋势均无显著性差异。低氧暴露前后,不同基因型携带者的ACE活性和AngⅡ水平差异不显著,A2350G位点和A1166C位点不同基因型组间的ACE活性变化量(△ACE(HY-NM))和AngⅡ水平变化量(△AngⅡ(HY-NM))差异也不,但A-240T位点不同基因型组间的ACE活性变化量(△ACE(HY-NM))差异显著。结论:1)血清ACE活性和AngⅡ水平不是急性低氧暴露的敏感指标。2)ACE基因A-240T、A2350G位点及AT1R基因A1166C位点多态性与AMS易感性及AMS评分变化趋势无关。A-240T位点与低氧暴露前后ACE活性变化量(△ACE(HY-NM))有关。
Objectives ： To study the change trend of the serum ACE activity and Ang Ⅱ concentration during the incidence of Acute Mountain Sickness （AMS） simulated by exercise in acute hypoxic exposure and the effects of polymorphisms of ACE and AT1 R on the serum ACE activity and Ang Ⅱ concentration. Methods ：49 students, all male Chinese Han origin lowlanders, were involved in a 6h acute exposure to 4800m altitude equivalent. Within 30 min of arriving at altitude, volunteers exercised at constant work rate for 20min on a cycle ergometer, then rested for the remainder of the 6h altitude exposure. Incidence and severity of AMS symptoms were determined by using the LLS. During the normoxic resting （NM） and at the end of the acute hypoxic exposure （ HY）, serum ACE activity and Ang Ⅱ concentration were analyzed. PCR - RFLP was used to determine the genotypes and alleles frequencies of ACE at A -240T, A2350G loci, as well as of AT1R at A1166C loci. Results ： 1 ） The serum ACE activity increased slightly in the AMS group after hypoxia exposure, while it decreased slightly in the Non AMS group. No significant differences of the ACE concentration change volume （△ ACE （ HY - NM） ） were found between the two groups. The Ang Ⅱ concentrations of the two groups decreased,but in the AMS group, it decreased significantly;In the Non AMS group, it decreased unremarkably. No significant differences of the Ang Ⅱ concentration change volume （ △ Ang Ⅱ （ HY - NM） ） were found between the two groups. 2） At the loci of ACE and AT1 R, there were no remarkable differences of the AMS accidences, and the AMS score change trends in different genotypes and alleles after hypoxia exposure. Before and after hypoxia exposure, no significant differences of the ACE activity, and Ang Ⅱ concentration were detected in different genotypes. ACE activity change volumes （ △ ACE （ HY - NM） ） were remarkably different in different genotypes at loci A - 240T of ACE, while no significant differences of the ACE activity change volumes （ △ ACE （ HY - NM） ） and the Ang Ⅱ concentration change volumes （△ Ang Ⅱ（ HY - NM） ） were found in different genotypes, either at loci A2350G of ACE or at loci A1166C of AT1R. Conclusions： 1 ） Serum ACE activity and Ang Ⅱ concentration are not sensitive marks for the acute hypoxia exposure. 2） A -240T, A2350G, as well as Al166C loci. were associated with the incidence of AMS and AMS score change trend. A - 240T loci of ACE was associated with the ACE change volume （ △ACE （ HY - NM） ） of hypoxia exposure.
出处
《山东体育学院学报》
北大核心
2014年第2期63-69,共7页
Journal of Shandong Sport University
基金
北京市教委重点实验室开放性课题
