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细胞毒性淋巴细胞相关抗原4融合蛋白对大鼠肝移植急性排斥反应及CD4^+、CD8^+T淋巴细胞的影响

Effect of cytotoxic T lymphocytic associated antigen 4 on acute rejection and CD4^+ , CD8 ^+ T cells after liver allotransplantation in rats
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摘要 目的观察应用细胞毒性淋巴细胞相关抗原4融合蛋白(CTLA4Ig)对大鼠肝移植术后对CD4^+、CD8^+T细胞的影响。方法建立大鼠肝移植急性排斥反应模型,随机分为A组:对照组,B组:实验组,CTLA4Ig80μg腹腔注射。检测B7-1和B7-2mRNA的表达,检测CD4^+、CD8^+T淋巴细胞浸润,确定排斥反应。结果B组2、3、7dB7—1和B7—2表达均较A组降低(B7—1:0.166±0.012比0.353±0.043;0.375±0.022比0.503±0.058;0.475±0.023比0.799±0.135;P〈0.01;B,7-2:0.170±0.022比0.341±0.040;0.357±0.031比0.475±0.041;0.512±0.103比0.829±0.124;P〈0.01)。B组2、3、7dCD4^+、CD8^+T淋巴细胞浸润均较A组降低(CD4^+:12.7±3.7比21.0±4.4:15.5±3.8比28.2±5.2;18.6±4.0比35.0±7.0;P〈0.01;CD8^+:9.3±1.5比16.9±2.9;12.1±2.2比19.9.4-5.3;17.1±3.7比29.0±5.4;P〈0.01);B组2、3、7dRAI均较A组降低(1.3±0.2比1.9±0.3,P〈0.05;2.0±0.4比2.6±0.5,P〈0.05;2.5±0.7比4.6±1.1,P〈0.01)。B组大鼠的生存期较A组明显延长[(40.9±9.3)d比(9.2±2.1)d,P〈0.01]。结论应用CTLA41g可以诱导大鼠肝脏移植免疫耐受。 Objective To observe the effects of cytotoxic T lymphocytic associated antigen 4 (CTLA4Ig) on CD4^ + and CD8^ + T cells after orthotopic liver transplantation in rats (ROLT). Methods The ROLT was established. All rats were divided into 2 groups randomly ( group A : control group, and group B: study group). CTLA4Ig was administrated by intraperitoneal injection at a dose of 80 μg. B7 expression was detected. CD4^+/CD8^+T cells were measured. RAI was identified. Results The expression of BT-1 and B7-2 mRNA was decreased significantly on the 2nd, 3rd, and 7th day in group B as compared with group A ( B7-1 : 0. 166 ± 0. 012 vs. 0. 353 ± 0. 043 ; 0. 375 ± 0. 022 vs. 0. 503 ± 0. 058 ; 0. 475 ± 0. 023 vs. 0.799±0.135;P〈0.01; B7-2:0.170±0.022 vs. 0.341 ±0.040; 0.357 ±0.031 vs. 0.475± 0. 041 ; 0. 512 ± 0. 103 vs. 0. 829 ± 0. 124 ; P 〈 0. 01 ). The infiltration of CD4 ±/CD8 ± T cells was decreased significantly on the 2nd, 3rd, and 7th day in group B as compared with group A ( CD4 ± 12. 7 ± 3.7 vs. 21.0±4.4; 15.5 ±3.8 vs. 28.2 ±5.2; 18.6 ±4.0 vs. 35.0±7.0;P〈0.01; CD8±: 9.3 ± 1.5 vs. 16.9±2.9; 12.1 ±2.2 vs. 19.9 ±5.3; 17.1±3.7 vs. 29.0±5.4;P〈0.01). The RAI was decreased significantly on the 2nd, 3rd, and 7th day in group B as compared with group A ( 1.3 ±0. 2 vs. 1.9±0.3,P〈0.05; 2.0±0.4 vs. 2.6±0.5,P〈0.05; 2.5 ±0.7 vs. 4.6±1.1,P〈0.01). Survival period was significantly longer in group B than in group A [ (40. 9 ± 9. 3 ) d vs. ( 9. 2 ± 2. 1 ) d, P 〈 0. 01 ]. Conclusion Rat liver allograft immune tolerance can be induced by CTLA4Ig.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2014年第5期1009-1011,共3页 Chinese Journal of Experimental Surgery
关键词 肝脏移植 细胞毒性淋巴细胞相关抗原4融合蛋白 CD4^+ CD8^+T细胞 性排斥反应 Liver transplantation Cytotoxic T lymphocytic associated antigen 4 CD4 ^+ / CD8 ^+ T cells Acute rejection
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