摘要
目的探讨活血温肾方对肝纤维化大鼠的防治作用及作用机制。方法采用四氯化碳肝纤维化大鼠模型,随机分为五组:正常组、模型组及三个用药组(温肾组、活血组、合用组),用药组分别予以温肾方、活血方、合用方(温肾方+活血方)灌胃,连续用药4周后,ELISA法测定大鼠血清中透明质酸(HA)、睾酮(T)、雌二醇(E2),比色法测定ALT、AST、A、G、肝组织羟脯氨酸(HYP),计算脏器指数。结果①各用药组的ALT、AST,HA及肝组织HYP含量均降低,逆转A/G倒置,温肾组及合用组优于活血组,尤以合用组效果最好。②各组之间T及E2含量差异有统计学意义(P<0.05),各组T含量依次为:合用组>温肾组>正常组>活血组>模型组。各组E2含量依次为:模型组>活血组>正常组>温肾组>合用组。③温肾组及合用组的睾丸脏器指数升高,肾脏指数在各组之间差异无统计学意义。病理学显示,各用药组大鼠肝纤维化与模型组相比均有不同程度的改善,且以合用组最为显著。结论活血温肾法具有较好的抗肝纤维化作用,可能是通过纠正性激素的紊乱而发挥作用的。从而为临床治疗肝纤维化提供了新思路、新方法和新药物。
Object To investigate the effect and mechanism of Kidney-Warming Decoction on CCL4-induced fibrosis of the liver in rats.Methods Using CCL4-induced fibrosis of the liver in rats,randomly divided into the normal group,the model group,kidney-warming group,promoting blood flow group and combination group.The latter three groups were intragastric administration with Kidney-Warming Decoction,Promoting Blood Flow Decoction and Combination Decoction.After 4 weeks,detected hyaluronic acid （HA）,T,E2 by ELISA,determined the ALT,AST, A,G,hydroxyproline （HYP）of liver tissue by colorimetric method,and calculated visceral index.Results ①The ALT,AST,HA and HYP concentration of three treatment groups were decreased,reversal of white/ball upside down.The effect of kidney-warming group and combina-tion group were better than promoting blood flow group,the combination group was the best.②Testosterone （T）and estradiol （E2 ）concentra-tion of each group had significant difference （P〈0. 05）.T concentration in order：kidney-warming group〉combination group〉normal group〉promoting blood group〉model group;E2 concentration in order：model group〉promoting blood group〉normal group〉combination group〉kidney-warming group.③Testis index of kidney-warming group and combination group were increased and renal index was no significantly differ-ent between groups.Pathology showed that the degree of liver fibrosis in medication group rats was lower than mode group ,and combination group was the most significant.Conclusion It is suggested that Kidney-Warming Decoction had good effect on treating hepatic fibrosis,may be through adjusting sex hormones disorder.It provides new ideas,new methods and new drugs for the clinical treatment of hepatic fibrosis.
出处
《中国中医药现代远程教育》
2014年第8期154-156,共3页
Chinese Medicine Modern Distance Education of China
关键词
温肾
活血
肝纤维化
大鼠
Kidney-Warming Decoction
hepatic fibrosis
hydroxyproline
sex hormone
rats
