期刊文献+

药物干预对低能量激光视网膜损伤后NMDAR表达的影响

Effect of MK-801 on the expression of glutamate receptor after laser-induced eye injury
下载PDF
导出
摘要 目的观察MK-801对低强度激光视网膜照射后谷氨酸受体(NMDAR)表达的拮抗作用,探讨MK-801治疗激光视网膜损伤的可能机制。方法用氦氖激光造成大鼠视网膜损伤模型,照后即刻肌注MK-801(2 mg/kg),通过免疫组化和原位杂交等方法,观察MK-801对激光照射后视网膜细胞中NMDAR蛋白和mRNA表达变化的影响。结果免疫组化和原位杂交的结果显示,激光照射后视网膜中NMDAR的表达显著增加,与视网膜的损伤程度相一致,照后给予MK-801可明显地减少视网膜中NMDAR的表达。结论谷氨酸受体的高表达可能是激光致眼损伤的机制之一,而MK-801可以通过抑制其表达来拮抗低强度激光所导致的视网膜损伤。 Objective To observe the effect of MK-801 on the expression of glutamate receptor after laser irradiation and to explore the possible mechanism of therapeutic effect of MK-801 on laser-induced retina injury. Methods After irradiation with low power He-Ne laser for 15 mins, Wistar rats immediately received an intramuscular injection of MK-801. The curative effects of MK-801 were observed by detection of the expression of glutamate receptor (NMDAR) in the rat retina. Results Laser irradiation induced a remarkable increase of NMDAR expression which corresponded with the retina injury process. However, the level of NMDAR expression decreased dramatically after administration of MK-801. Conclusion High expression of NMDAR is a possible mechanism through which laser cause experimental retinal injury. MK-801 showed the neuroprotective effect due to its blocking effect on NMDAR expression.
出处 《首都公共卫生》 2014年第2期56-59,共4页 Capital Journal of Public Health
基金 国家自然科学基金资助项目(81000685 81072239) 北京市自然科学基金资助项目(7142124)
关键词 激光 视网膜 损伤 NMDAR MK-801 Laser Retina Injury NMDAR MK-801
  • 相关文献

参考文献12

  • 1Chung H, Dai T, Sharma SK, et al. The nuts and bolts of low-level laser (light) therapy. Annals Biomed Eng, 2012, 40(2): 516-533.
  • 2Ferraiuolo L, Kirby J, Grierson AJ, et al. Molecular pathways of motor neuron injury in amyotrophic lateral sclerosis. Nat Rev Neurol, 2011, 7( 11): 616-630.
  • 3Izumi Y, Shimamoto K, Benz AM, et al. Glutamate transporters and retinal excitotoxicity. Glia, 2002, 39 ( 1 ) : 58 - 68.
  • 4梁洁,陈鹏,王少霞,方毅,康宏向,高荣莲,王长振,姜严明.低强度激光视网膜损伤效应的实验研究[J].应用激光,2012,32(6):548-551. 被引量:5
  • 5Sattler R, Tymianski M. Molecular mechanisms of glutamate receptor-mediated excitotoxic neuronal celldeath. Mol Neurobiol, 2001, 24(1 -3): 107-129.
  • 6Park KW, Nozell SE, Benveniste EN. Protective role of STAT3 in nmda and glutamate-induced neuronal death: negative regulatory effect of SOCS3. PloS one, 2012, 7 ( 11 ) : e50874.
  • 7Nguyen D, Alavi MV, Kim KY, et al. A new vicious cycle involving glutamate excitotoxicity, oxidative stress and mitochondrial dynamics. Cell Death Dis, 2011, 2 (12): e240.
  • 8Zhang C, Du F, Shi M, et al. Ginsenoside Rd Protects Neurons Against Glutamate-Induced Excitotoxicity by Inhibiting Ca^2+Influx. Cell Mol Neurobiol, 2012, 32( 1 ) : 121 - 128.
  • 9Shen Y, Liu XL, Yang XL. N-methyl-D-aspartate receptors in the retina. Mol Neurobiol, 2006, 34(3) : 163 - 179.
  • 10Izumi Y, Hammerman SB, Kirby CO, et al. Involvement of glutamate in ischemic neurodegeneration in isolated retina. Visual Neurosci, 2003, 20(2) : 97 - 107.

二级参考文献9

  • 1王晓英,单清,马萍,陈鹏,郭启煜,钱焕文.神经生长因子对激光致视网膜损伤后bFGF蛋白表达的影响[J].眼科研究,2007,25(4):277-280. 被引量:2
  • 2ELDER J A. Ocular effects of radiofrequency energy [J].Bioelectromagnetics, 2003, 6 (2) : 148-161.
  • 3GARRIGA P, MANYOSA J. The eye photoreceptor pro- tein rhodopsin. Structural implications for retinal disease [J]. FEBS Lett, 2002, 528 (1-3) : 17-22.
  • 4FLIESLER S J,ANDERSON R E. Chemistry and metabolism of lipids in the vertebrate retina [J]. Prog Lipid Res, 1983, 22 (2) : 79-131.
  • 5CHAHORY S,PADRON L,COURTOIS Y,et al. The LEI/L-DNase II pathway is activated in light-induced reti- nal degeneration in rats [J]. Neurosci Lett, 2004,367 (2) : 205-209.
  • 6KISELEV A, SOCOLICH M, VINOS J, et al. A molecular pathway for light-dependent photoreceptor apoptosis in Drosophila[J]. Neuron, 2000, 28 (1) 139-152.
  • 7BONFOCO E, KRALNC D, ANKARCRONA M,et al. Apoptosis and necrosis:two distinct events induced,respec- tively,by mild and intense insults with N-methyl-D-as- paxtate or nitric oxide/supperoxide in cortical cell cultures [J]. PNAS, 1995, 92 (16) 7162-7166.
  • 8陈鹏,康宏向,高荣莲,梁洁,肖瑞,马萍,钱焕文.KGF-2治疗激光角膜灼伤的形态计量学研究[J].首都公共卫生,2010,4(4):151-152. 被引量:4
  • 9陈鹏,姜严明,张杰,单清,马萍,张翠萍,钱焕文.Nd:YAG激光致视网膜感光细胞损伤及相关基因的表达[J].激光生物学报,2003,12(4):296-301. 被引量:9

共引文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部