摘要
目的探究三氧化二砷（As2O3）对侵袭相关因子β—catenin表达的影响是否与FOXO3a有关。方法采用脂质体转染siRNA以及LY294002药物改变大肠癌SW620细胞中FOXO3a的表达，Western blot检测FOXO3a、β—catenin蛋白表达水平，RT—PCR检测FOXO3a、β—catenin mRNA表达水平。结果As2O3增加SW620细胞中FOXO3a蛋白及mRNA的表达，同时降低β—catenin蛋白及mRNA表达（P〈0．05）。FOXO3a基因干扰后，β—catenin蛋白及mRNA的表达上调（P〈0．05）。LY294002药物处理后，β—catenin表达下调（P〈0．05）。结论As2O3对人大肠癌SW620细胞中β—catenin表达的抑制作用可能与其增强FOXO3a的活性有关。
Objective To investigate whether FOXO3a is involved in the changed expression of β - catenin in colorectal cancer SW620 ceils after exposure to arsenic trioxide （ As2O3 ）. Methods The expression of FOXO3a was changed by FOXO3a specific siRNA and LY294002. Then, the protein and mRNA expressions of FOXO3a and β- catenin in eoloreetal cancer SW620 cells were detected by western blot and RT -PCR, respectively. Results As2O3 can stimulate the protein and mRNA expressions of FOXO3a in SW620 cells, while reducing the expression of β - catenin at protein and mRNA levels （P 〈 0.05 ）. After FOXO3a gene silencing, the protein and mRNA expressions of β -catenin were obviously enhanced （ P 〈 0.05 ）. But LY294002 treatment could remarkably inhibit the expression of β - catenin at protein and mRNA levels. Conclusion The inhibitory effects of As2O3 on β - eatenin in eolorectal cancer SW620 cells may be associate with its ability to enhance the activity of FOXO3a.
出处
《徐州医学院学报》
CAS
2014年第4期258-261,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省高校自然科学基金（12KJD320006,07KJD310217）
徐州市科技局项目（XF11C1002011）
