摘要
运用Cre/LoxP位点特异性重组酶系统对特定组织和器官基因进行改造是遗传操作的有效手段,该系统可以将特定的DNA片段删除,研究特定基因在生长发育过程中的功能.研究Cre/LoxP系统的工作机制,比较眼内不同组织,如视网膜色素上皮（RPE）细胞、MUller细胞及感光细胞中特异性Cre工具鼠的作用特点,获得Cre转基因小鼠模型,从而建立合适的眼科实验动物模型,可为特定组织单基因缺失的功能研究提供实验基础.就Cre/LoxP系统、Cre转基因小鼠模型的获得和眼部特异性Cre重组酶模型小鼠的研究进展进行综述.
Cre/LoxP site specific recombinase system is an effective tool for gene recombination in the body to produce specific gene deletion or inversion.The study of gene function is the experimental basis for the development of experimental animal models.Study the Cre/LoxP system,compare the different function characteristics of mouse models for Cre recombinase system in the research of ophthalmology,epecially in the tissue of retina,such as retinal pigment epithelium （RPE） cells,MUller cells and photoreceptor cells,get the Cre transgenic mouse model to establish proper experimental animal models of ophthalmology will lay a solid foundation for the research of the lack of single gene for a particular organization study.Here,the research progress of Cre/LoxP system,the Cre transgenic mouse models and eye specific Cre recombinant enzyme model mice will be summarized.
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2014年第5期471-475,共5页
Chinese Journal Of Experimental Ophthalmology
关键词
转基因小鼠
动物模型
眼
Cre
Transgenic mouse
Animal model
Cre
Eye
