摘要
目的研究吴茱萸碱对涎腺腺样囊性癌ACC-M细胞侵袭的影响。方法用终浓度为0、0.01、0.1、1、10、100μg/ml的吴茱萸碱处理ACC-M细胞2、4、6、8、10 h后,应用transwell小室侵袭实验检测和观察ACC-M细胞的侵袭抑制情况。结果吴茱萸碱处理组较对照组穿过上室底面膜的细胞数少,细胞数随浓度增大,时间增长基本呈递减趋势,侵袭抑制率随浓度的增大而增大,随时间的增长而增大,侵袭抑制率最高可达59.99%。结论吴茱萸碱可以抑制涎腺腺样囊性癌ACC-M细胞侵袭。
Objective To investigate the effects of evodiamine on the invasive behaviors of salivary adenoid cystic carcinoma ACC-M cells.Methods ACC-M cells were treated with evodiamine at 0,0.01,0.1,1,10,100μg/ml for 2h,4h, 6h,8h,10h,respectively,then transwell invasion assay was used to examine the inhibition invasion condition of ACC -M cells. Results As compared with that in control group ,the number of the cell though the upper chamber in the evodiamine -treatment group was decreased.The cells＇number showed a gradual decreasing trend with concentration increasing and time prolonging . The invasion inhibition rate was increased with the increasing of drug concentration and action time .The highest invasion inhibition rate was 59.99%.There was a significant difference in number of the cell though the upper chamber among different concentration groups （ P 〈0.05）.Conclusion Evodiamine is able to inhibit the invasion of ACC -M cells.
出处
《河北医药》
CAS
2014年第9期1290-1292,共3页
Hebei Medical Journal
基金
河北省医学科学研究重点课题(编号:2005007)
河北省中医药管理局项目(编号:2006093)
关键词
吴茱萸碱
涎腺腺样囊性癌
侵袭
evodiamine
salivary adenoid cystic carcinoma
invasion
