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AngⅡ及其受体AT1在海水吸入型肺损伤中的作用及机制的研究 被引量:1

Angiotensin Ⅱ and its receptor AT1 contribute to seawater inhalation induced acute lung injury in rats
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摘要 目的 观察海水吸入型肺损伤大鼠肺组织中的血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)及其受体血管紧张素Ⅱ1型受体(angiotensinⅡtype 1 receptor,AT1)的变化情况,以及核因子κB(NF-κB)信号传导通路在海水吸入型肺损伤中的作用及其机制.方法 40只SD大鼠随机分为正常组、海水处理3h组、海水处理6h组和海水处理12 h组,每组10只,采用气管内滴注海水(3 ml/kg)的方法制造海水吸入型肺损伤模型.分别用放射免疫分析方法和ELISA法检测肺组织中AngⅡ、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和IL-1β的含量,并且用RT-PCR和Western blot方法检测肺组织中AT1和NF-κB的表达情况.结果 肺组织病理检查结果显示,不同时间的海水处理后可造成严重的肺损伤,其中海水处理6h后大鼠肺部损伤最严重.海水处理后AngⅡ的含量和AT1的表达明显升高;NF-κB在正常组表达很少,海水处理后明显升高.与此同时,IL-1β和TNF-α在海水处理3h后含量迅速增加,在海水处理6h后达高峰.结论 海水吸入可引起急性肺损伤,AngⅡ通过激活AT1和NF-κB引起炎症反应参与海水吸入型肺损伤的发生发展. Objective To observe the changes of angiotension Ⅱ (Ang Ⅱ) and angiotensin Ⅱ type 1 receptor (AT1) in seawater inhalation induced acute lung injury,as well as the role of nuclear factor-κB (NF-κB) pathway.Methods 40 Sprague Dawley rats were randomly divided into control group,seawater exposure 3 h group,seawater exposure 6 h group,and seawater exposure 12 h group.The lung injury model was established by endotracheal instillation of seawater (3 ml/kg).Subsquently,the expression of Ang Ⅱ was measured by radioimmunoassay,interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) by ELISA,AT1 and NF-κB by RT-PCR and Western blot.Results The lung pathology results showed that seawater aspiration might result in lung injury especially 6 h after intratracheal instillation of seawater.The expressions of Ang Ⅱ and AT1 were significantly up-regulated in the seawater inhalation induced acute lung injury groups compared to the control group,so was the change of NFκB.The levels of IL-1β and TNF-α increased rapidly at 3 h after seawater aspiration and peaked at 6 h.Conclusions Seawater aspiration can cause acute lung injury by up-regulating the AT1 and NF-κB induced inflammatory response,in which Ang Ⅱ may play a crucial role.
出处 《国际呼吸杂志》 2014年第10期736-740,共5页 International Journal of Respiration
基金 国家自然科学基金(81270124)
关键词 海水 急性肺损伤 血管紧张素Ⅱ 血管紧张素Ⅱ1型受体 核因子ΚB Seawater Acute lung injury Angiotensin Ⅱ Angiotensin Ⅱ type 1 receptor Nuclear factor-κB
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