摘要
建立喜树果和马比木中喜树碱的HPLC分析方法,并对喜树碱的含量进行比较。采用岛津LC-20A高效液相色谱系统对样品进行定量分析,色谱柱为ODS;甲醇∶水(5.5∶4.5)为流动相;检测波长为254nm;柱温35℃;流速0.8mL·min-1。该条件下目标组分与其他组分有效分离(R>1.5),喜树碱进样量在0.01-0.09μg线性范围关系良好,相关系数r=0.9998,平均回收率:99.98%,RSD:0.56%。该方法分离效果好、灵敏度高、分析速度快、操作简便、结果准确可靠。分析结果可为喜树果和马比木的深度开发提供参考依据。
Analysis method to establish the CPT fruit and mappia foetida HPLC, and the content of camptothecin were compared. Quantitative analysis was performed on the sample using the SHIMADZU LC-20A high performance liquid chromatography, Take PLA_TISILTM ODS（4.6×150mm, 5txm） as the column,methanol and water（55∶45）as the mobile phase.The inspect wavelengh isn 254nm;the column temperature is 30 and the velocity offlow is 0.8 mL omin-1;Under this condition, the target component and other components of effective separation（R 1.5） the lineal range is 0.01-0.09. The coefficient of correlation, r =0.9998, recovery rate：99.98%, RSD： 0.56%. This method, with the advantages of good separation effect, specificity, high sensibility,rapid analysis, The analytic result would provide reference for deep development of camptothecine and mappia foetida.
出处
《食品与发酵科技》
CAS
2014年第2期89-91,共3页
Food and Fermentation Science & Technology
基金
绵阳市科技局项目(13G002-2
12C003-3)
四川省教育厅科研项目(12ZA079
12ZA081)
关键词
喜树果
马比木
喜树碱
HPLC
fruit of camptothecine acuminata decne
appia foetida
campthothecin
HPLC
