摘要
干旱是影响我国绿豆生产的主要因素之一,筛选抗旱性种质资源,培育抗旱品种,对我国绿豆产业发展具有重要意义。本研究首先以4份绿豆种质为材料,通过检测5个梯度渗透势PEG-6000溶液模拟旱胁迫下的发芽率动态分布,确定了绿豆芽期模拟旱胁迫的适宜PEG-6000溶液渗透势为-0.9 MPa(21.8%)。进而以-0.9 MPa的PEG-6000溶液对113份绿豆种质进行芽期抗旱鉴定,通过测定发芽势、发芽率、下胚轴长、胚根长、干重和鲜重等10项指标及相关性分析,认为相对发芽势、相对发芽率、相对下胚轴长、相对胚根长、相对总芽长、相对鲜重、相对干重、相对发芽指数、相对活力指数9项指标可以作为评价绿豆芽期抗旱性指标。利用隶属函数分析法,对113份种质的芽期抗旱性进行综合评价,筛选出1份高抗种质当地吉豆(C0000626)和16份抗性种质,为绿豆抗旱基因发掘及抗旱品种改良奠定基础。
Drought stress is a major problem for mungbean production and the drought tolerance evaluation is a priority research for mungbean in whole world. The germination rate of 4 accessions of mungbean germplasm resources was tested with 5 PEG-6000 concentrations to determine the proper PEG-6000 solution for drought resistance screening. The results showed that 21. 8%（-0. 9 MPa） of PEG-6000 was the suitable concentration for screening drought resistant resources at bud stage for mungbean. 113 accessions of mungbean germplasms resources were evaluated the drought resistance with-0. 9 MPa PEG-6000 solution. Correlation analysis results showed that relative values of germination energy,germination rate,hypocotyl length,radical length,total length,fresh biomass, dry biomass,germination index,and vigor index were closely related to the drought resistance. Based on the subordinative function analysis of screening results,one germplasm,Dangdijidou（ C0000626） with the most drought resistance and 16 drought resistance germplasms were identified and recommended to be used in the drought tolerance improvement for mungbean breeding.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第3期498-503,共6页
Journal of Plant Genetic Resources
基金
国家现代农业产业技术体系建设专项(CARS-09)
中国农业科学院科技创新工程
科技支撑计划(2013BAD05-2-4)
关键词
绿豆
PEG-6000
芽期
抗旱性
相关性分析
隶属函数
mungbean
PEG-6000
bud stage
drought resistance
correlation analysis
subordinative function analysis
