摘要
表面等离子体共振免疫传感器是一类相对新型的免疫检测技术。将链霉亲和素 生物素系统用于表面等离子体共振免疫传感的信号放大 ,实时检测了人免疫球蛋白G(hIgG)的蛋白浓度。发生免疫反应的传感片和生物素化抗体反应后 ,传感片表面的一层生物素分子随后与链霉亲和素 生物素化抗体复合物中的链霉亲和素的活性位点发生亲和反应 ,从而使传感片表面特异健合的物质质量显著增加 ,大大提高了免疫检测的灵敏度和检测限。免疫反应经放大后 ,可检测 0 0 0 5～ 10
Surface plasmon resonance(SPR)immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years.However,a major disadvantage of SPR for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly.Therefore,it is a challenging task to develop strategies for improving the detection limit/sensitivity of SPR.In this paper,authors present a novel strategy for improving the sensitivity of SPR immunosensing using streptavidin biotinylated antibody complex.It is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity.This amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein.The complex can be formed in a cross linking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第2期186-191,共6页
Acta Scientiarum Naturalium Universitatis Pekinensis
基金
中国博士后科学基金
中国科学院王宽诚博士后工作奖励基金资助项目
