摘要
目的探讨非粒细胞缺乏侵袭性肺曲霉病(IPA)患者血清中IL-17、Dectin-1水平的变化及其意义。方法分别收集23例确诊及临床诊断为IPA的非粒细胞缺乏患者(IPA组)、31例临床诊断为肺炎患者(肺炎组)和51例健康体检者(对照组)的资料,应用ELISA法测定血清IL-17含量;同时检测IPA组及对照组血清Dectin-1含量,并测定IPA组患者血清G试验、GM试验、外周血白细胞计数和血清CRP的水平,随访患者预后,分析血清IL-17与其相关性。结果 IPA组患者血清IL-17含量明显高于对照组(P<0.05);肺炎组患者血清IL-17水平与对照组差异无统计学意义(P>0.05);IPA组血清Dectin-1含量明显高于对照组(P<0.05);IPA组血清IL-17与Dectin-1水平呈正相关(r=0.81,P<0.05);IPA组血清IL-17、Dectin-1水平与G试验、GM试验、白细胞、CRP及患者的预后无相关性(P>0.05)。结论非粒细胞缺乏宿主曲霉感染时刺激Dectin-1产生,激活Th17细胞免疫反应,且两者间存在相关性。
Objective To explore the changing levels of serumIL-17 and Dectin-1 and their implication in non-neutropenic patients with invasive pulmonary aspergillosis（IPA）.Methods The clinical data were reviewed for 23 non-neutropenic patients with clinical diagnosis of IPA（IPA group）,31 patients with clinical diagnosis of pneumonia（pneumonia group） and 51 healthy subjects（control group）.The peripheral serum was collected to analyze IL-17 level by ELISA.Serum Dectin-1 level was also determined at the same time.Serum G test,GM test,WBC and CRP level were also assayed for the patients in IPA group.Patient outcome was followed up and analyzed in terms of serum IL-17.Results The serum IL-17 level of the patients in IPA group was significantly higher than that in the control group（P〈0.05）.The serum IL-17 level in the pneumonia group was not significantly different from that in the control group（P〉0.05）.The serum Dectin-1 level in IPA group was significantly higher than that in the control group（P〈0.05）.For the patients in IPA group,serum IL-17 level was positively correlated to serum Dectin-1 level（r= 0.81,P〈0.05）,while serum IL-17 and Dectin-1 levels were not correlated to the results of G test,GM test,WBC,CRP or patient outcome（P〉0.05）.Conclusions Dectin-1 may be produced and Th17 cell immunity activated in response to Aspergillus fumigatus infection in non-neutropenic patients.And,serum Dectin-1level is correlated with Th17 response.
出处
《中国感染与化疗杂志》
CAS
北大核心
2014年第3期240-244,共5页
Chinese Journal of Infection and Chemotherapy
基金
国家自然科学基金-青年科学基金(81000003)
南京军区南京总医院院管课题(2011028)
