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大鼠广泛肠切除后小肠组织miR-125a和Mcl-1的表达及意义

Expression and significance of miR-125a and Mcl-1 in intestinal tissue after massive small bowel ;resection in rat
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摘要 目的:探讨广泛肠切除后miR-125a和抗凋亡蛋白(Mcl-1)在肠上皮细胞中表达的变化及其在短肠综合征剩余肠管适应性变化过程中的意义。方法选取8周龄的SD雄性大鼠54只,分为实验组(18只,进行70%的大范围小肠切除,距回盲部近端15 cm和Treitz韧带远端10 cm处分别切断小肠,然后行空回肠端端吻合)、肠切除对照组(18只,在距回盲部近端15 cm处切断肠管然后再吻合)和手术对照组(18只,仅行开关腹手术)。术后1周在距吻合口远端1 cm处取材,采用免疫组织化学方法和实时荧光定量 PCR检测各组大鼠小肠组织中 Mcl-1和 miR-125a 表达。结果实验组大鼠肠组织中Mcl-1阳性表达率为18.8%(3/16),明显低于手术对照组(76.5%,13/17)和肠切除对照组(83.3%,15/18)(均P<0.01);miR-125a相对表达量为1.92,明显高于肠切除对照组(1.01)和手术对照组(1.05)(均P<0.01)。结论 miR-125a和Mcl-1在广泛肠切除诱导的肠道适应过程中发挥重要作用,二者之间通过一定的机制相互调节。 Objective To investigate the expression and significance of miR-125a and anti-apoptotic protein Mcl-1 in intestinal tissue after massive small bowel resection in intestinal adaptation. Methods Sprague-Dawley rats (54 male rats, 8-week old) were divided into 3 groups randomly, including two control groups. Rats in the experiment group were subjected to 70% massive small bowel resection. Rats in the resection group underwent simple intestinal resection and anastomosis. Rats in the control group underwent laparotomy alone. A 5 cm intestine approximately 1 cm distal to the anastomosis was harvested a week after operation. Expression of Mcl-1 was assessed by immunohistochemistry and real-time PCR was used to detect the expression of miR-125a in intestinal tissue. Results The positive expression of Mcl-1 in the experiment group was 18.8%(3/16), significantly lower than that in the control group(76.5%, 13/17) and the resection group (83.33%, 15/18)(both P〈0.01). The expression of miR-125a in the experiment group was 1.92, significantly higher than that in the control group(1.01) and the resection group (1.05)(both P〈0.01). Conclusion miR-125a and anti-apoptotic protein Mcl-1 may play an important role in intestinal adaptation process and they may regulate each other through a certain pathway.
出处 《中华胃肠外科杂志》 CAS CSCD 2014年第5期495-498,共4页 Chinese Journal of Gastrointestinal Surgery
基金 广东省科技计划项目(2011B031800271) 大亚湾科技计划项目(2013A01015)
关键词 肠切除术 短肠综合征 MCL-1 MCL-1 miR-125a Massive small bowel resection Short bowel syndrome miR-125a
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  • 1Xin Zhou1,Yuan Xin Li2,Ning Li2,Jie Shou Li2 1Department of General Surgery, Medical School, Nanjing University, Nanjing 210093. Jiangsu Province. China2Research Institute of General Hospital. Chinese PLA General Hospital of Nanjing Military Area, Nanjing 210002. Jiangsu Province. China.Effect of bowel rehabilitative therapy on structural adaptation of remnant small intestine: animal experiment[J].World Journal of Gastroenterology,2001,7(1):66-73. 被引量:14
  • 2Rossi L,Kadamba P,Hugosson C,et al.Pediatric short bowel syndrome:adaptation after massive small bowel resection.J Pediatr Gastroenterol Nutr,2007,45(2):213-221.
  • 3Byrne TA,Morrissey TB,Nattakom TV,et al.Growth hormone,glutamine,and a modified diet enhance nutrient absorption in patients with severe short bowel syndrome.JPEN J Parenter Enteral Nutr,1995,19(4):296-302.
  • 4Den Hond E,Geypens B,Ghoos Y.Effect of high performance chicory inulin on constipation.Nutr Res,2000,20(5):731-736.
  • 5Jeppesen PB.Growth factors in short-bowel syndrome patients.Gastroenterol Clin North Am,2007,36(1):109-121.
  • 6Wapnir RA,Wingertzahn MA,Teichberg S.L-arginine in low concentration improves rat intestinal water and sodium absorption from oral rehydration solutions.Gut,1997,40(5):602-607.
  • 7Thomas S,Anup R,Susama P,et al.Nitric oxide prevents intestinal mitochondrial dysfunction induced by surgical stress.Br J Surg,2001,88(3):393-399.
  • 8Hwang JM,Chan DC,Chang TM,et al.Effects of oral arginine and glutamine on radiation-induced injury in the rat.J Surg Res,2003,109(2):149-154.
  • 9Schleiffer R,Raul F.Prophylactic administration of L-arginine improves the intestinal barrier function after mesenteric ischaemia.Gut,1996,39(2):194-198.
  • 10Osowska S,Moinard C,Neveux N,et al.Citrulline increases arginine pools and restores nitrogen balance after massive intestinal resection.Gut,2004,53(12):1781-1786.

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