摘要
目的 研究尾悬吊模拟失重对大鼠胃窦肌层诱生型环氧化酶2（Cox-2）表达和血清生长抑素（SS）、血管活性肠肽（VIP）水平的影响。方法 健康雄性Wistar大鼠64只，随机分为8组，按模拟失重时相分为0h（对照组）和6h、12h、1d、2d、3d、5d、7d组（n=8）。采用尾悬吊法建立模拟失重动物模型。应用RT-PCR、Western blotting和免疫组化技术检测各组大鼠胃窦肌层组织中Cox-2 mRNA和蛋白表达情况，应用放免法测定血清SS、VIP浓度。结果 免疫组化结果显示，Cox-2蛋白阳性表达为棕褐色，悬吊6h、12h、1d大鼠胃窦平滑肌细胞胞质染色变深并出现部分核染色阳性，2d开始逐渐恢复，但胞质着色仍较对照组深。RT-PCR结果显示，Cox-2 mRNA表达水平在尾悬吊6h即出现显著增高，12h、1d时仍呈高水平表达（P＜0.05），2d后表达水平逐渐回落（P＞0.05）。Western blotting结果显示，悬吊6h大鼠胃窦肌层Cox-2蛋白表达水平显著升高，12h、1d持续高表达，与对照组比较差异有统计学意义（P＜0.05），2d后逐渐恢复正常水平（P＞0.05）。血清SS浓度在尾悬吊模拟失重2-3d达峰值，与对照组比较差异有统计学意义（P＜0.05），其他各时相浓度值波动明显。血清VIP浓度同样在模拟失重早期即显著升高（P＜0.05），峰值出现在6-12h，其后经过2-3d的回落后复又升高。结论 胃窦肌层Cox-2表达异常和血清SS及VIP水平变化在胃窦失重应激性动力障碍及恢复过程中可能发挥重要作用。
Objective To investigate the e？ect of simulated weightlessness on cyclooxygenase-2 （Cox-2） expression in gastric antrum muscle layer as well as levels of serum somatostatin （SS） and vasoactive intestinal peptide （VIP） in rats. Methods Sixty four adult male Wistar rats were randomly divided into eight groups （n=8）, namely, suspended for 6h, 12h, 24h, 2d, 3d, 5d, 7d and 0h （control group）. Tail-suspension was used to reproduce the weightlessness animal model. mRNA and protein expression of Cox-2 in rats＇ gastric antrum muscle layer were assayed by RT-PCR, Western blotting and immunohistochemistry. Serum concentrations of SS and VIP were determined by radioimmunoassay. Results The immunohistochemistry assay showed that Cox-2 protein was stained as brown particles, and the cytoplasm of smooth muscle cells was deeply stained at 6-, 12- and 24-h suspension with partial positive nuclear staining. Some lessening was observed after 2-d suspension, but the cytoplasm staining was still deeper than that of control. RT-PCR showed that the Cox-2 mRNA in gastric antrum muscular layer was significantly up-regulated at 6 h, and it remained so after 12 and 24h of suspension compared with the control group （P〈0.05）, followed by a lowering after 2-d suspension. Western blotting showed that the level of Cox-2 protein expression was also elevated, and returned to normal after 2 days. The concentration of SS increased significantly after 2-3d of suspension （P〈0.05）, and it showed a marked fluctuation in other groups. The level of serum VIP also increased significantly in the early phase of suspension, with a peak value at 6-12h （P〈0.05）, and it was followed by a lowering in 2 to 3 days, but it was increased again. Conclusion Changes in Cox2 expression in gastric antrum muscle layer and serum SS and VIP concentrations may play an important role in the occur of stress-reactive gastric dynamics disorder and the process of recovery in rats under tail-suspension simulated weightlessness.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2014年第5期383-387,共5页
Medical Journal of Chinese People's Liberation Army
基金
全军医学科研"十二五"重点项目(BWS11J051)~~
关键词
失重模拟
环氧化酶2
生长抑素
血管活性肠肽
weightlessness simulation
cyclooxygenase-2
somatostatin
vasoactive intestinal peptide
