摘要
钙库操作性钙离子通道(store-operated calcium entry,SOCE)是介导胞外Ca2+进入细胞内的重要通道之一,其核心蛋白由位于内质网上的基质相互作用分子(stromal interaction molecule,STIM)和位于细胞膜上的Orai蛋白构成。目前研究发现,STIM蛋白存在STIM1和STIM2两种亚型,其主要功能略有不同。当内质网内钙库中Ca2+消耗之后,STIM蛋白通过其特殊的结构能够感受内质网内钙库中Ca2+浓度的变化,发生快速的转位和聚合化等激活反应,与质膜上的Orai蛋白偶联,实现SOCE通路的功能开放,引起Ca2+内流。当钙库中Ca2+得到补充之后,STIM蛋白与Orai蛋白缓慢解离即失活,通路关闭。目前对STIM蛋白结构的研究提示,通过其激活和失活机制不仅能够参与调节SOCE通路的开放与关闭,也参与对细胞内重要的细胞增殖、分化等功能活动调控。STIM蛋白可能成为治疗多种疾病的潜在的新靶点。
Store-operated calcium entry （SOCE） is one of the important channels mediating extracellular Ca^2＋ entry. Stromal interaction molecule （STIM） proteins in the membrane of endoplasmic reticulum and Orai proteins in the plasma membrane are two key components of SOCE. Studies have presented two homologues of STIM --STIM1 and STIM2, with slight difference in function. After the depletion of Ca^2＋ store, STIM undergoes rapid multimerization and translocation to interact and activate Orai by its specific structures which sense the change of Ca^2＋store to activate and open SOCE channel for inward Ca^2＋influx. After restoring of Ca^2＋ STIM slowly dissoci- ates with Oral and becomes inactive to shut the channel. In current studies about STIM structure and function, it is suggested that STIM is involved in the regulation of cellular functions： proliferation, differentiation, etc via turning active or inactive to regulate the open and shut of SOCE channel. The novel functions of STIM predict that it has a great potential to become new target of diseases.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2014年第5期686-690,共5页
Chinese Journal of Cell Biology
基金
重庆市自然科学基金(批准号:CSTC2010BB5031)
第三军医大学回国人员启动基金(批准号:2011XHG01)
国家自然科学基金(批准号:31271467)资助的课题~~
