摘要
目的:探讨钙网织蛋白(calreticulin,CRT)在骨肉瘤组织中的表达与骨肉瘤生长、转移的关系,以及化疗对其表达的影响。方法:取2005-10-01-2010-09-31在山东中医药大学附属医院(15例)、山东大学齐鲁医院(11例)及新汶矿务局集团中心医院(4例)骨科行骨肉瘤切除术、病历资料完整并排除合并其他运动系统肿瘤的骨肉瘤标本30例,其中转移20例,术前化疗者20例。每例标本同时取距离瘤组织>30mm的正常组织作对照。免疫组织化学染色法检测CRT在瘤体组织和正常组织中的表达;RT-PCR法检测CRT在mRNA水平的表达;蛋白质印迹法检测CRT蛋白的表达。结果:CRT主要存在于细胞质内,在所有标本中都有表达。其表达强度为瘤体组织(1.07±0.32)<瘤体周围正常组织(2.68±0.09),P<0.01;肿瘤转移组(0.93±0.21)<未转移组(1.36±0.42),P=0.020;术前未化疗组(0.75±0.21)<术前化疗组(1.24±0.30),P=0.001。CRT mRNA的表达强度为瘤体组织(0.58±0.30)<瘤体周围正常组织(1.48±0.14),P<0.01;肿瘤转移组(0.50±0.22)<未转移组(0.73±0.23),P=0.033;术前未化疗组(0.27±0.13)<术前化疗组(0.74±0.23),P<0.01。CRT蛋白的表达水平为瘤体组织(369.35±14.27)<瘤体周围正常组织(599.23±6.78),P<0.01;肿瘤转移组(361.95±17.37)<未转移组(394.15±17.97),P=0.031;术前未化疗组(288.96±12.86)<术前化疗组(409.54±18.22),P<0.01。结论:CRT的表达在人骨肉瘤组织中降低,其mRNA和蛋白质表达水平下降程度与瘤体是否转移有关,化疗可增加其表达水平。
OBJECTIVE：To detecte the expression of Calreticulin（CRT）in patients with osteosarcoma and analyzed the expression difference in tumor tissue and normal tissue,pre-and post-chemotherapy,metastasis and non-metastasis. According to these results,we analyzed the relationship between the expression of CRT and tumor growth,metatasis,and chemotherapeutics.METHODS：The specimens from 30patients with osteosarcoma were collected as experimental group, in which 10cases were non-metastasis,20were metastasis,and 20were pre-chemotherapy,10were post-chemotherapy. Thirty specimens from normal tissues were collected as control group.The specimens were detected by immunofluorescence staining.Total RNA was extracted from crushed tissue which including experimental and control group,and tested by RT-PCR method.The protein was extracted from crushed tissue including experimental and control group,and analyzed by Western-Blot.RESULTS：CRT was mainly located in intracavity of cell endoplasmic reticulum.The expression level of CRT in normal tissue（2.68±0.09）was higher than that in tumor tissue（1.07±0.32,P〈0.01）,the expression level of CRT was higher in non-metastasis group（1.36±0.42）than that in metastasis group（0.93±0.21,P=0.020）,and the expression level of CRT was higher in post-chemotherapy group（1.24±0.30）than that in pre-chemotherapy group（0.75±0.21,P=0.001）.The expression level of CRT mRNA in normal tissue（1.48±0.14）was higher than that in tumor tissue（0.58±0.30,P〈0.01）,in non-metastasis group（0.73±0.23）it was higher than in metastasis group（0.50± 0.22,P=0.033）and in post-chemotherapy group（0.74±0.23）it was higher than in pre-chemotherapy group（0.27± 0.13,P〈0.01）.The expression level of CRT protein in normal tissue（599.23±6.78）was higher than that in tumor tissue（369.35±14.27,P〈0.01）,the expression level was higher in non-metastasis group（394.15±17.97）than that in metastasis group（361.95±17.37,P=0.031）,and the expression level of CRT in post-chemotherapy group（409.54± 18.22）was higher than that in pre-chemotherapy group（288.96±12.86,P〈0.01）.CONCLUSION：The expression level of CRT in normal tissue around the tumor is higher than that of tumor tissue,in non-metastasis group it is higher than that of metastasis group,post-chemotherapy group is higher than that of pre-chemotherapy group.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2014年第10期757-761,共5页
Chinese Journal of Cancer Prevention and Treatment
