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高地芽孢杆菌PY41碱性蛋白酶纯化 被引量：1

Separation and purification of alkaline protease from Bacillus altitudinis PY41
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摘要 该文对高地芽孢杆菌(Bacillus altitudinis)PY41菌株的碱性蛋白酶进行了分离纯化,并对其酶学性质进行了研究。通过硫酸铵分级沉淀、凝胶过滤层析和疏水层析得到纯化的碱性蛋白酶,经SDS–PAGE凝胶电泳检测得到该蛋白酶相对分子质量为29.5 kDa。 To purify alkaline protease of Bacillus altitudinis PY41,and study its properties. By using（NH4）2SO4 segmentation precipitation,Sepharose CL–6B and Phenyl Sepharoses to purify the alkaline protease of Bacillus altitudinis PY41,the protease was determined by SDS–PAGE to have a molecular weight of 29.5 kDa.
作者 李文 陈复生 丁长河 李盘欣
机构地区 河南工业大学粮油食品学院 河南南街村集团有限公司技术中心
出处 《粮食与油脂》 北大核心 2014年第5期38-40,共3页 Cereals & Oils
基金 国家863计划(2013AA102208) 河南省成果转化资金项目(132201610014) 郑州市创新型科技人才队伍建设工程
关键词 高地芽孢杆菌 碱性蛋白酶 纯化 Bacillus altitudinis alkaline protease purification
分类号 TS201.25 [轻工技术与工程—食品科学]
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参考文献12

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二级参考文献45

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粮食与油脂
2014年 第5期

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