摘要
目的:优选生脉分散片的提取纯化工艺。方法:以提取物中人参皂苷Rg1,Re含量及浸膏得率的综合评分为指标,采用正交试验考察乙醇体积分数、溶剂用量、提取时间对提取工艺的影响;采用壳聚糖絮凝沉降法,通过正交试验考察提取液浓缩程度、壳聚糖用量、静置时间对纯化工艺的影响。结果:最佳提取工艺为加8倍量85%乙醇回流提取3次,每次2 h;人参皂苷Rg1,Re质量分数分别为0.251%,0.074%。最佳纯化工艺为提取液生药质量浓度1 g·mL-1,壳聚糖用量10%,静置时间12 h;人参皂苷Rg1,Re质量分数分别为0.394%,0.116%。结论:优选的提取纯化工艺稳定合理,为生脉分散片的剂型改革提供参考。
Objective: To optimize extraction and purification processes of Shengmai dispersible tablets. Method: Taking composite score of contents of ginsenoside Rg1 , Re and yield of extract as index, orthogonal design was adopted to investigate effects of ethanol concentration, solid-liquid ratio, extracting time on extraction process; Chitosan flocculation sedimentation method was employed, orthogonal test was adopted to optimize purification technology with concentration degree of extracting solution, dosage of ehitosan, standing time as factors. Result: The best extraction process was as follows: extracted thrice with eight times the amount of 85 % ethanol for 2 h each time; contents of ginsenoside Rg1 and Re were 0. 251% and 0. 074%. Optimum purification process was as following: the concentration of extracting solution 1 g ·mL^-1 , dosage of chitosan 10% , standing time for 12 h; contents of ginsenoside Rg1 and Re were 0. 394% and 0. 116%. Conclusion: This optimized process was stable and feasible for providing a reference to dosage-form reformation of Shengmai dispersible tablets.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第11期41-44,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南中医学院研究生创新项目(2012YCX001)