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RP-HPLC波长切换法同时测定妇康片中3种成分的含量 被引量:3

RP-HPLC with UV Switch Determination of Three Active Components in Fukang Tablet
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摘要 目的:建立RP-HPLC同时测定妇康片中芍药苷、延胡索乙素及阿魏酸含量的方法。方法:采用SepaxC18(4.6mm×250mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸(B)为流动相,梯度洗脱,流速1.0mL·min^-1,检测波长230nm(0~16min,芍药苷)、280nm(16.1~25min,延胡索乙素)、320nm(25.1~50min,阿魏酸),柱温30℃。结果:芍药苷、延胡索乙素、阿魏酸分别在0.242~2.42,0.02~0.203,0.045~0.448pLg(r≥0.9996)呈良好的线性关系,平均回收率为99.75%,100.33%,100.45%,RSD分别为0.61%,1.46%,1.73%。结论:该HPLC方法简单,灵敏度高,可用于同时测定妇康片中芍药苷、延胡索乙素及阿魏酸的含量。 Objective: To establish a simultaneous determination method for paeoniflorin, tetrahydropalmatine and ferulie acid in Fukang tablet. Method:. The determination was performed on a Sepax C18 column with acetonitrile-0.1% ammoniae aqua as the mobile phase. The'detection wavelength were at 230 nm (0-16 min, paeoniflorin), 280nm (16. 1-25 min, tetrahydropalmatine) and 320 nm (25.1-50 min, ferulie acid), the flow rate was 1.0 mL· min^-1. The column temperature was 30 ℃. Result: The paeoniflorin, tetrahydropalmatine and ferulic acid presented a good linearity in the concentration range of 0. 242-2.42, 0.02- 0. 203, 0. 045-0. 448 ixg (r≥0. 999 6) , The average rate of recovery was 99.75% , 100.33% and 100. 45% with RSD =0. 61% , 1.46% , 1.73% (n =6) respectively. Conclusion: This method was simple, accurate and reproducible, it can be used to determine the content of paeoniflorin, tetrahydropalmatine and ferulic acid in Fukang tablet.
出处 《中国实验方剂学杂志》 CAS 北大核心 2014年第11期58-61,共4页 Chinese Journal of Experimental Traditional Medical Formulae
基金 赣州市指导性科技计划项目(201308128)
关键词 妇康片 芍药苷 延胡索乙素 阿魏酸 Fukang tablet paeoniflorin tetrahydropalmatine ferulic acid
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