摘要
本研究以拟南芥为试验材料,探索基因at5g66070在受到非生物胁迫的表达情况,并研究其抗逆特性.结果表明,在NaCl处理下,该基因的表达量明显升高,表明该基因受到盐的诱导.通过PCR和RT-PCR在DNA和RNA水平上筛选鉴定at5g66070基因T-DNA插入纯合突变体.分析了野生型拟南芥与突变体在不同浓度NaCl处理下,其萌发率,根长以及苗期的生长差异.结果发现,相对于野生型来说,突变体对盐更敏感.具体来说,在含有150mMNaCl的MS培养基中,突变体的萌发率比野生型要低,根长比野生型要短,同时突变体幼苗更容易出现枯萎,萎黄症状.
In this work, the expression and the function of gene at5g66070 in various abiotic stress were studied. The results showed that the gene expression was significantly increased after NaCl treatment, indicating that the gene was induced by salt stress on the other hand. One homozygous T-DNA mutant was identified at DNA and RNA levels by PCR and RT-PCR. Then the germination rate, root length and seedling growth were analyzed between wild-type and mutant under the treatment of different NaCl concentrations. The results showed that the mutant is more sensitive to salt compared to the wide-type. The mutant＇s germination rate is lower ,and their root lengths were shorter than that of wt Meanwhile, the mutant seedlings were more prone to be chlorosis when they are growing on the MS medium with 150mM NaCl.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第3期615-620,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(31171586)
转基因重大专项(2011ZX08009-003-002)
中央高校基本科研业务费(2011SCU11107)
