摘要
为研究自拟中草药组合物防控鸡新城疫病毒的效果,提取组方的可溶性成分制成1.0g/mL的组合物,采用体外、体内2种方式研究其抗病毒作用。以预防、直接、治疗3种方式将组合物(以生理盐水作为对照)与病毒注入鸡胚,检测病毒效价,并计算保护率;制备新城疫病理模型,将SPF鸡分为预防+治疗组、治疗组灌服组合物,测定发病率、发病保护率、死亡率、死亡保护率。结果显示,与对照组相比,组合物3种作用方式均有抑制鸡胚新城疫病毒的作用,以预防方式抑制作用最强,能有效降低病毒4.35个滴度、减少病毒量62.23%,对鸡胚的保护率达100%;预防+治疗组发病保护率提高92.5个百分点、死亡率降低30.0个百分点。可见,自拟中草药组合物对新城疫病毒具有较强的抑制作用。
Provention and control effects of the self-made Chinese herbal medicine composition on newcastle virus were examined.The soluble components of composition were extracted and concentrated to 1 g/mL.The antiviral effect was tested by two ways,in vitro and in vivo.In order to confirm antiviral titer and protective rate,the mixture of extract and newcastle virus was injected into the egg with three ways of prevention,interreaction and treatment,compared with the control of physiological saline.The pathological model of newcastle disease was prepared.The pathological models were drenched the extract of 1 g/mL concentration with two groups of prevention＋ treatment group and treatment group to evaluate the antiviral effects by mortality ratio,deathprotection ratio and incidence ratio,onset-protection ratio compared with the control.The results showed that prevention＋treatment group had the best role than those which were three ways of prevention,interreaction and treatment,reduced 4.35 titles and 62.23% the amount of virus,the protective rate reached 100%.The way of prevention＋treatment group had high protect affects compared with the control group,mortality rate decreased 30.0 percentage points and protection rate increased 92.5 percentage points.The extraction of Chinese herbal medicine by self-made has powerful inhibition effect on newcastle virus.
出处
《河南农业科学》
CSCD
北大核心
2014年第5期156-159,共4页
Journal of Henan Agricultural Sciences
基金
郑州市科技发展计划项目(2001NYXM229)
关键词
中草药组合物
新城疫病毒
鸡胚
病理模型
Chinese medicine herbs mixture
newcastle virus
chicken egg
pathologic model
