摘要
目的 :探讨高效扩增腺病毒载体的方法。 方法 :用人胚胎肾 2 93细胞株扩增重组腺病毒 ,并测定病毒滴度。 结果 :扩增得到腺病毒的滴度约为 5× 10 8PFU/ ml。 结论 :用人胚胎肾 2 93细胞株扩增重组腺病毒 ,在不进行任何浓缩时可达较高病毒滴度 ,为腺病毒介导的基因转移方法提供了理论基础。
Objectives:To investigate the method of generating recombinant adenovirus vector containing human p16(Ad p16) efficiently. Methods:Recombinant adenovirus vector was generated with HEK 293 cells, and the titer of the adenovirus was detected. Results:The titer of generated adenovirus is about 5×10 8 PFU/ml. Conclusions:Recombinant adenovirus vector was generated with HEK 293 cells with high titer. This set the foundation of adenovirus mediated gene transfer.
出处
《医学研究生学报》
CAS
2001年第1期44-46,共3页
Journal of Medical Postgraduates
