摘要
选取 1 994年以来从中国部分地区分离的 8株新城疫病毒 ( NDV)野外毒株 ,用反转录聚合酶链式反应 ( RT PCR)技术扩增其 F基因重要的功能区片段 ,同时进行克隆和序列测定 ,构建 NDV的遗传进化树 ,分析其遗传关系 .结果表明 :所分离的毒株在分裂位点的氨基酸顺序均相当于 NDV的强毒株 .通过遗传进化树分析表明 8株分离株中有 7株为基因 VII型新城疫病毒 ,说明基因
Part sequence of fusion gene (from 47 to 581 nt) of 8 NDV strains isolated from 7 provinces between 1994 to 2000 were amplified by RTPCR and further cloned into pGEM RT vector. The sequence analysis showed that the length of these F gene segments all were 535 bp. The amino acid sequence of the cleavage site region was 112 RRQ/RKRF 117 , compatible to virulent NDV strains. A phylogenetic tree based on obtained sequences of reference NDV strains was constructed. The result that most of the isolates belonged to genotype Ⅶ indicated that the genotype Ⅶ NDV was the major pathogeny causing the epizootic of ND in China in recent years.
出处
《扬州大学学报（自然科学版）》
CAS
CSCD
2001年第1期35-40,共6页
Journal of Yangzhou University：Natural Science Edition
