依那普利对百草枯中毒大鼠肺组织ACE表达的影响被引量：2

Changes of ACE Expression in Rat Lung Following Paraquat Poisoning and Intervention Effects of Enalapril

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摘要目的：观察依那普利对百草枯（PQ）中毒大鼠肺组织病理改变、血管紧张素转化酶（ACE）表达的影响。方法一次性PQ 60 mg/kg灌胃建立大鼠百草枯中毒肺纤维化模型，干预组染毒后予依那普利10 mg/（kg·d）灌胃。于染毒后3 d、7 d、14 d取肺组织行HE染色和Masson染色，观察大鼠肺泡炎、肺纤维化程度；Quantitative Real-Time PCR检测ACE mRNA表达。结果干预组在染毒后3d、7d肺泡炎症减轻；染毒7d、14d后，纤维化程度积分低于模型组。模型组染毒后各时间点大鼠肺组织ACE mRNA表达逐步上调，持续至14 d。干预组各时间点ACE mRNA低于模型组。结论依那普利可能通过降低局部ACE表达，从而减轻百草枯中毒所致肺纤维化形成。 Objective To investigate the effects of enalapril on histopathologic alteration and the expression of angiotensin converting enzyme （ACE） gene in lung of paraquat （PQ） poisoning rats. Methods A rat model of pulmonary ifbrosis was induced by intragastric administration with a single dose （60 mg/kg） of paraquat. Then intervention group was treated intragastrically with enalapril at a dose of 10 mg/（kg·d）. Rats were sacriifced and lungs were harvested at different time of day 3, day 7 or day 14 following poisoning. The level of pulmonary al-veolitis and ifbrosis were estimated by using HE staining and Masson staining. The expression of ACE mRNA was measured by quantitative Real-Time PCR. Results Compared with model group, intervention group exhibited signs of less serious pulmonary alveolitis on day 3 and day 7. Similarly, intervention group brought a lower ifbro-sis scores on day 7 and day 14. After PQ exposure, lung ACE mRNA expression observed in model group were gradually up-regulated at each time point and were maintained until day 14. Expression levels of ACE mRNA in lung tissue of intervention group were lower than those of model group at all time points. Conclusion Enalapril could alleviate paraquat-induced pulmonary ifbrosis, which may be due to suppressing ACE mRNA expression in local tissue.

作者陆件沈奕庄智伟鲍逸民

机构地区苏州市立医院本部急诊科

出处《中国血液流变学杂志》 CAS 2013年第4期609-611,630,共4页 Chinese Journal of Hemorheology

关键词依那普利百草枯中毒肺纤维化血管紧张素转化酶 enalapril paraquat poisoning pulmonary ifbrosis angiotensin converting enzyme

分类号 R595.4 [医药卫生—内科学]

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1Senarathna L, Eddleston M, Wilks MF, et al. Prediction of outcome after paraquat poisoning by measurement of the plasma paraquat concentration[J]. QJM, 2009, 102(4): 251 - 259.
2Yamashita M, Yamashita M, Ando Y. A long-term follow- up of lung function in survivors of paraquat poisoning[J]. Hum Exp Toxicol, 2000, 19(2): 99 - 103.
3Szapiel SV, Elson NA, Fulmer JD, et al. Bleomycin- induced interstitial pulmonary disease in the nude, athymic mouse[J]. Am Rev Respir Dis, 1979, 120(4): 893 - 899.
4Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4): 402 - 408.
5Dinis-Oliveira RJ, Duarte JA, Sfinchez-Navarro A, et al. Paraquat poisonings: mechanisms of lung toxicity, clinical features, and treatment[J]. Crit Rev Toxicol, 2008, 38(1):13-71.
6Matej R, Housa D, Pouckova P, et al. Radiation-induced production of PAR-1 and TGF-beta 1 mRNA in lung of C57B16 and C3H murine strains and influence of pharmacoprophylaxis by ACE inhibitors[J]. Pathol Res Pratt, 2007, 203(2): 107 - 114.
7Shenoy V, Ferreira A J, Qi Y, et al. The angiotensin- converting enzyme 2/angiogenesis-(1-7)/Mas axis confers cardiopulmonary protection against lung fibrosis and pulmonary hypertension[J]. Am J Respir Crit Care Med, 2010, 182(8): 1065 - 1072.