摘要
目的 探讨葛根素注射液对大鼠视网膜光损伤的修复作用及其机理.方法 SD大鼠48只随机分为正常组、模型对照组、高剂量治疗组和低剂量治疗组,除正常组外,各组大鼠接受22000±1000 lux手术显微镜光源持续照射30分钟,治疗组于照射后第二天连续给药一周后,处死所有动物,观察各组间视网膜组织形态学改变和细胞色素c（Cyt-c）,Caspase-3表达的差异.结果 ① 组织形态学上,正常组大鼠视网膜形态正常,模型组视网膜外核层变薄,细胞排列较正常组稀疏,内外节排列紊乱,分界不清;低剂量治疗组视网膜形态有改善,高剂量治疗组较模型组无明显改善.② 高剂量组Cyt-c、Caspase-3表达有一定下调,但与模型组比较差异无统计学意义（P〉0.05）,低剂量组Cyt-c、Caspase-3的表达显著下调,与模型组比较差异有统计学意义（P〈0.05）.结论 低剂量葛根素注射液能一定程度减轻模型大鼠视网膜光损伤,促进光损伤后视网膜修复,其作用机理可能与调节视细胞凋亡相关基因,一定程度抑制视细胞凋亡有关.
Objective To observe the morphologic,biochemical and electrophysiologic changs of the retina in rats after exposure to the light of the operation microscope, and study the protective effect and mechanism of Pue against photic injury of retina. Methods The experiments were performed on fouty-eight healthy Sprague-Dawley rats which were devided into four groups-normal group, model group, high-dose group and low-dose group. All the groups except the normal one were continually exposed to the light of the operation microscope with an illuminace level of 22000 q-10001ux for 30 minutes. The treated received daily Pue for one week after exposure to the light, then, the pathological changes of retina were examined by light microscopy;and INOS,Cyt-c and Caspase-3 into retina were tested. Results （1） After the light exposure,the outer nucleus layer of retina in the model group became thinner and the regular arrangement of outer and inner segments were disordered, dividing line wasn＇t distinct. In the low-dose group the outer nucleus layer of retina got thicker than that of the model group. （2）Pue can adjust and control the gene expression in phototonus of retina. Conclusions Pue could release the photic injury of retina,accelerate the renovation of retina after light exposure and ameliorate the function of retina. The mechanism might relate to alleviate the poisonous effect of retina that nitric oxide result in,control the gene expression in phototonus of retina.
出处
《中医眼耳鼻喉杂志》
2012年第1期21-23,共3页
Journal of Chinese Ophthalmology and Otorhinolaryngology
