摘要
目的 建立以高效液相色谱法测定复方盐酸普鲁卡因溶液III中盐酸普鲁卡因含量的方法.方法 采用高效液相色谱法,用Ultimate LP-C18色谱柱（4.6 mm×0 mm,5 μm）,以0.1%庚烷磺酸钠的0.05 mol·L-1磷酸二氢钾溶液（用磷酸调pH至3.0）-甲醇（68∶ 32）为流动相,流速为1.0 mL·min-1,检测波长为290 nm.结果 盐酸普鲁卡因线性范围为10.66～106.6 μg·mL-1（r=0.999 7）,平均加样回收率为99.2%（RSD=0.8%）.结论 该方法快速、方便,操作简单,其他成分无干扰,适用于测定复方盐酸普鲁卡因溶液III中盐酸普鲁卡因的含量.
Objective To establish an HPLC method for the determination of procaine hydrochloride in Compound Pro- caine Hydrochloride Solution III. Methods The HPLC was conducted on a Uhimate LP - C18 column（4. 6 mm × 250 mm, 5μm）with 0. 05mol· L-1 KH2PO4 0. 1% heptane sulfonic acid sodium salt（adjusted to pH 3 with phosphoric acid） - methanol （ 68：32 ） as mobile phase, the flow - rate was 1.0 mL· min -1, the detection wavelength was 290 nm. Results The linear ranges of procaine hydrochloride were 10. 66 - 106. 6 μg·mL-l （ r = 0. 999 7 ） , the average recovery rate was 99.2% ,RSD =0. 8% （n =6）. Conclusion The method was simple,sensitive,convenient,and suitable for the determina- tion of procaine hydrochloride in Compound Procaine Hydrochloride Solution III.
出处
《药学研究》
CAS
2013年第9期513-514,共2页
Journal of Pharmaceutical Research
关键词
高效液相色谱
盐酸普鲁卡因
HPLC
Compound Procaine Hydrochloride Solution III
Procaine hydrochloride
