摘要
[目的]研究肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis-inducing ligand,TRAIL)对人肺腺癌细胞株Calu-3增殖和凋亡的影响。[方法]用不同浓度TRAIL分不同时间段干预Calu-3细胞,MTT法检测细胞增殖抑制率,流式细胞仪检测细胞凋亡率。[结果]Calu-3细胞生长被TRAIL抑制,具有浓度和时间依赖性。TRAIL能诱导Calu-3细胞凋亡。[结论]TRAIL在体外具有抑制Calu-3细胞增殖,促使Calu-3细胞凋亡的作用。
[ Purpose ] To investigate the effect of TNF-related apoptosis inducing ligand （TRAIL） on the proliferation and apoptosis of the human lung adenocarcinoma cell line Calu-3. [Methods ] Calu ceils were treated with different concentrations of TRAIL at different times. Inhibition of proliferation and apoptosis of Calu-3 cells were detected by MTY assay and flow cytometry. [Results] The proliferation of Calu-3 cells were inhibited by TRAIL in a dose-time dependent manner. Apoptosis of Calu-3 cells was induced by TRAIL. [Conclusion] TRAIL could inhibit Calu-3 cells proliferation and induce cell apoptosis in vitro.
出处
《肿瘤学杂志》
CAS
2014年第5期369-372,共4页
Journal of Chinese Oncology
基金
国家自然科学基金(81274002)
福建省自然科学基金(2012J01383)
福建省自然科学基金面上项目(2010J01222)
