摘要
为了探讨肌醇对哲罗鲑（Hucho taimen）生长性能、体成分及消化酶活性的影响，以鱼粉、明胶和酪蛋白为蛋白源配制肌醇含量为99.8（不添加肌醇）、199.8、299.8、499.8、699.8、899.8和5099.8 mg/kg的7种饲料，分别投喂7个处理组，每组3个重复，每重复30尾鱼，进行为期56 d的饲养实验。结果表明，投喂肌醇含量为499.8 mg/kg饲料组的增重率最大，且与添加量低于299.8 mg/kg的3个饲料组差异显著（＜0.05）。饲料系数在499.8 mg/kg饲料组最低，在5099.8 mg/kg饲料组最高，两组间差异显著（＜0.05）。肌醇含量为499.8~899.8 mg/kg饲料组的特定生长率显著高于99.8~299.8 mg/kg及5099.8 mg/kg饲料组（＜0.05）。肌肉中水分、粗脂肪和粗蛋白各组间差异不显著（＞0.05），肝淀粉酶、幽门盲囊脂肪酶活性各组间差异不显著（＞0.05）。肠道蛋白酶活性在肌醇含量为199.8~499.8 mg/kg饲料组较高，显著高于其他组（＜0.05），脂肪酶活性在299.8~899.8 mg/kg饲料组高于99.8 mg/kg饲料组（＜0.05）。由此得出，饲料中适量添加肌醇可提高哲罗鲑的生长性能及消化酶活性；肌醇含量过低时哲罗鲑增重率、消化酶活性均较低；肌醇含量过高时哲罗鲑特定增长率、消化酶活性较低，饲料系数较高；以增重率为指标由折线回归模型分析得出，肌醇的最适含量为536.6 mg/kg饲料。
Hucho taimen is a fast growing, easily domesticated, carnivorous fish that is valued for its taste and nutri-tional value. This species has been domesticated and bred successfully in more than a dozen provinces in China, in-cluding Heilongjiang, Liaoning, Shandong, and Xinjiang. Despite prior research, the nutrient requirements of H. taimen are poorly understood. We evaluated the effects of dietary myo-inositol （MI） levels on body composition, growth per-formance, and digestive enzyme activity in H. taimen. We formulated seven semipurified basal diets using fish meal, gelatin, and casein as the protein source. The MI content of the diets was 99.8, 199.8, 299.8, 499.8, 799.8, 899.8, or 5 099.8 mg MI/kg diet. Each diet was fed to triplicate groups of 30 fish （mean initial weight：2.83＆#177;0.44 g） for 56 d. The weight gain rate was highest in fish fed a diet with 499.8 mg MI/kg and was significantly higher （〈0.05） than that in fish fed diets with≤299.8 mg MI/kg. Weight gain rate was lowest in the group fed a diet with 99.8 mg MI/kg. Feed conversion ratio was lowest in H. taimen fed a diet with 499.8 mg MI/kg, and was significantly （〈0.05） lower than in H. taimen fed a diet with 299.8 mg MI/kg or 5 099.8 mg MI/kg. The feed conversion ratio was highest in fish fed a diet with 5 099.8 mg MI/kg. The specific growth rate was significantly higher （〈0.05） in fish fed 499.8-899.8 mg MI/kg than fish fed diets with≤200 or 5 099.8 mg MI/kg. Supplementation of dietary myo-inositol did not affect moisture, crude lipid, crude protein, or amylase activity in the liver and lipase activity in the pyloric caeca （〉0.05）. Fish fed a diet with 499.8 mg MI/kg had the highest （〈0.05） protease activity in the liver, followed those fed by 699.8 mg MI/kg. Lipase activity increased initially and then decreased as the level of dietary MI increased. Lipase activity in the liver of H. taimen fed 299.8 mg MI/kg was significantly （〈0.05） higher than in those fed 99.8 mg MI/kg. Protease activity was significantly higher （〈0.05） in the intestine of fish fed diets with 199.8-499.8 mg MI/kg than those fed the other diets. Lipase activity was significantly higher in the intestine of fish fed 299.8-899.8 mg MI/kg than fish fed 99.8 mg MI/kg. Amylase was significantly higher （〈0.05） in the intestine of fish fed 499.8 mg MI/kg than in the remaining groups. In conclusion, supplementation of diets with moderate concentrations of myo-inositol resulted in better growth perform-ance and digestive enzyme activity in H. taimen. Feeding diets with lower MI concentrations was associated with lower weight gain, specific growth rate, crude lipid levels, and digestive enzyme activity. Feeding diets with excessive MI was associated with reduced growth performance, digestive enzyme activity and feed conversion efficiency. Based on weight gain rate using broken-line regression, the weight gain rate in H. taimen was maximized by feeding 536.6 mg MI/kg diet. Our results provide insight into the dietary MI requirements for H. taimen.
出处
《中国水产科学》
CAS
CSCD
北大核心
2014年第3期560-566,共7页
Journal of Fishery Sciences of China
基金
农业部公益性行业专项(201003055)
中央级公益性科研院所基本科研业务费项目(201103)
关键词
哲罗鲑
肌醇
生长性能
体成分
消化酶活性
Hucho taimen
myo-inositol
growth performance
body composition
digestive enzyme activity
