摘要
目的探讨5-氟尿嘧啶(5-FU)联合人参皂苷Rg3对胃癌细胞增殖的抑制作用。方法 MTT法检测5-FU联合Rg3对人胃癌细胞MGC-803和MKN-28细胞增殖的抑制作用。构建体外肿瘤球模型研究5-FU联合人参皂苷Rg3对肿瘤球的生长抑制作用;流式细胞仪检测5-FU联合人参皂苷Rg3对MKN-28细胞的凋亡诱导作用。构建胃癌裸鼠移植瘤模型,随机分为生理盐水组(阴性对照组)、5-FU组、Rg3组和5-FU+Rg3组(联合给药组);研究5-FU联合人参皂苷Rg3对裸鼠移植瘤的生长抑制作用,统计各组肿瘤的大小,绘制肿瘤生长曲线。结果 MGC-803胃癌细胞和MKN-28胃癌细胞给药48 h后,联合给药组细胞存活率显著低于其他组,差异具有统计学意义(P<0.01)。MGC-803胃癌细胞肿瘤球和MKN-28胃癌细胞肿瘤球给药7 d后,联合给药组肿瘤球体积显著小于其他组,差异具有统计学意义(P<0.01)。细胞凋亡实验结果显示:联合给药组诱导肿瘤细胞凋亡能力显著强于其他组,差异具有统计学意义(P<0.01)。荷瘤裸鼠实验结果显示:各给药组均能有效抑制肿瘤的生长,与阴性对照组比较差异具有统计学意义(P<0.01)。联合给药显著延长荷瘤裸鼠的中位生存期,差异具有统计学意义(P<0.01)。结论 5-FU与Rg3均具有抗胃癌细胞的作用,二者联合应用是一种潜在的治疗胃癌的有效手段。
Objective To investigate the inhibitory effect of 5-fluorouracil （5-FU） combined with Rg3 on the proliferation of gastric cancer cells. Methods The inhibitory effect of 5-FU combined with Rg3 on the proliferation of human gastric cancer cells MGC-803 and MKN-28 was investigated by the MTT assay. The in vitro tumor spheroid model was built for studying the inhibitory effect of 5-FU combined with Rg3 on the growth of tumor spheroid. The gastric xenograft tumor model of nude mice was built and mice were randomly divided into the saline group （ negative control group）, 5-FU group, Rg3 group, and 5-FU ＋ Rg3 group （ combined administration group）. The inhibitory effect of 5-FU combined with Rg3 on the growth of xenograft tumor of nude mice was investigated. Tumor sizes of each group were measured and growth curves of tumors were plotted. Results The viability of MGC-803 cells and MKN-28 cells of the combined administration group was significantly lower than that of the other groups after being administrated by 5-FU and Rg3 for 48 h. Thedifference was statistically significant （P〈0.01）. The sizes of tumor spheroid of MGC-803 cells and MKN-28 cells of the combined administration group were significantly smaller than those of the other groups after being administrated by 5-FU and Rg3 for 7 d. The difference was statistically significant （P〈0. 01）. The results of apoptosis tests showed that the ability of inducing apoptosis of the combined administration group was significandy stronger than that of the other groups. The difference was statistically significant （P〈0.01）. The results of tumor bearing nude mice tests showed that each administration group could inhibit the growth of tumors. Compared to the negative control group, the differences were statistically significant （P〈0.01）. The combination of 5-FU and Rg3 could prolong the median survival time of tumor bearing nude mice. The difference was statistically significant （ P〈0.01）. Conclusion Both 5-FU and Rg3 can inhibit the growth of gastric cancer cells and the combination of 5-FU and Rg3 may be a potentially effective treatment method for gastric cancer.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2014年第5期645-649,共5页
Journal of Shanghai Jiao tong University:Medical Science
