摘要
背景:虽然对于单一组织的冷冻保存获得了空前的成果,并且已逐渐应用于临床,但是对于复合组织的冷冻保存及应用还鲜有研究。目的:通过实验研究深低温冷冻不同复温方法下兔肢体再植后血管形态学变化,找出一种对复合组织中血管损伤最小的复温方法,从而为深低温处理后断肢再植可行性提供理论依据。方法:30只健康新西兰大白兔随机数字表法均分为对照组、慢速冷冻-慢速复温组、慢速冷冻-快速复温组,均给予大白兔右后肢自膝上1 cm处离断。慢速冷冻两组复温后均行断肢再植,再植肢体成活6 h后给予再次离断右后下肢。3组兔断肢均取股血管组织采用染色光镜及电镜进行形态学观察及大体观察,光镜病理计分结果用显著性分析。结果与结论:慢速冷冻-慢速复温组、慢速冷冻-快速复温组兔肢体再植6 h后的病理变化(大体标本、光镜、电镜)均较对照组差,但慢速冷冻-慢速复温组与慢速冷冻-快速复温组相比血管内皮细胞完整性较好,细胞器破坏较少。证实,经过深低温冷冻-复温处理后兔断肢再植6 h,离断肢体血管组织能保持一定的结构完整性,再植后6 h兔肢体获得成活,且慢速冷冻-慢速复温组更为适合离断肢体的保存,为深低温处理后离断肢体行断肢再植远期成活可行性提供了依据。
BACKGROUND：The cryopreservation of single tissue has achieved great advancement and is gradual y applied in clinics. However, the cryopreservation of complex tissue is rarely reported.
OBJECTIVE：To investigate the morphological change in rabbit limb tissue after replantation through different rewarming methods, find the best rewarming methods of compound textured blood vessels, and provide theoretical basis for the feasibility of limb replantation after long-term cryopreservation.
METHODS：Thirty New Zealand white rabbits were randomly divided into control group, slow freezing-slow thawing group, and slow freezing-rapid thawing group. The right posterior limbs of al the rabbits were cut off 1 cm above the knee joint. Except control group, the latter two groups were given limb replantation after thawing, and then the right posterior limb was again cut off after the replanted limbs were survived for 6 hours. For al groups, the histological changes and gross observation in aorta tissue were observed by light microscopy and transmission electron microscope, and the results were analyzed with statistical methods.
RESULTS AND CONCLUSION：In the slow freezing-slow thawing, slow freezing-rapid thawing groups, the pathological changes （gross specimen, light microscope, electron microscope） of rabbit limbs 6 hours after replantation were worse than those in control group. Compared with slow freezing-rapid thawing group, better integrity of endothelial cells and less damage of the organel es were found in slow freezing-slow thawing group. Through deep cryogenic freezing-thawing process, rabbit limb blood vessels can maintain the structural integrity after replantation and survived at 6 hours. Slow freezing-slow thawing is better than slow freezing-rapid thawing for the preservation of severed limbs, providing evidences for the long-term survival fol owing a deep cry ogenic treatment after the severed limb replantation.
出处
《中国组织工程研究》
CAS
CSCD
2014年第15期2357-2362,共6页
Chinese Journal of Tissue Engineering Research
