摘要
目的探讨清除腹腔巨噬细胞(peritoneal macrophages,PMs)对重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠肠道屏障功能的影响。方法 36只SD大鼠随机分为PMs保留组、PMs清除组和假手术组,每组12只。PMs保留组与PMs清除组经胆胰管逆行注射质量分数2%去氧胆酸钠建立大鼠SAP模型,假手术组注射生理盐水。PMs清除组分别于造模前5d、前2d腹腔注射氯屈磷酸二钠脂质体清除PMs,PMs保留组与假手术组同样时间注射等量空脂质体。检测3组血清淀粉酶及血浆D-乳酸水平,比较胰腺病理评分、肠道转运系数及脏器细菌移位率。结果 PMs保留组与PMs清除组血清淀粉酶水平((9 399±923)、(9 012±852)u/L)高于假手术组((1 367±360)u/L)(P<0.01),PMs保留组与PMs清除组比较差异无统计学意义(P>0.05);假手术组大鼠肠道转运系数(0.46±0.12)高于PMs清除组(0.33±0.10)与PMs保留组(0.23±0.09)(P<0.01),PMs清除组高于PMs保留组(P<0.05);假手术组大鼠脏器细菌移位率((5.60±0.68)%)、血浆D-乳酸水平((5.66±0.43)mg/L)低于PMs清除组(40.55±5.67)%,(8.96±0.67)mg/L)与PMs保留组((51.63±7.53)%,(12.62±0.89)mg/L))(P<0.01),PMs清除组低于PMs保留组(P<0.05);PMs清除组胰腺病理评分(7.9±1.2)与PMs保留组(7.6±0.9)比较差异无统计学意义(P>0.05)。结论造模前PMs消耗对SAP大鼠肠道屏障功能有保护作用。
Objective To observe the effect of depleting peritoneal macrophages （PMs） on intestinal barrier function damage in rats with severe acute pancreatitis （SAP）. Methods Thirty-six SD rats were randomly divided into PMs- preserving group, PMs-depleting group and sham operation group, with 12 rats in each group. PMs-preserving group and PMs depleting group were injected retrogradely 2% deoxysodium cholate into cholangiopancreatic duct to establish SAP rat models, and sham operation group was injected 0.9% NaCl instead of deoxysodium cholate. PMs-depleting group was injected liposome Cl2 MBP into peritoneal cavity to deplete PMs, and PMs-preserving group and sham operation group were injected the same volume of 0.9% NaC1 on the 5th and 2nd day before the establishment of SAP models. The levels of serum amylase and Dqactic were detected, and the pancreatic pathologic score, intestinal transfer coefficient and the rate of organ bacterial translocation were compared among three groups. Results The level of amylase was significantly lower in sham operation group （（1 367± 360） u/L） than that in PMs-preserving group （（9 399± 923）u/L and PMs- depleting group （ （ 9 012 ± 852） u/L） （P〈 0.01 ）, and there was no significant difference between PMs-preserving group and PMs-depleting group （P〉0.05）. The intestinal transfer coefficient was significantly higher in sham operation group （0.46±0.12） than that in PMs-preserving group （0.23±0.09） and PMs-depleting group （0.33±0.10） （P〈0.01）, and was significantly higher in PMs-depleting group than that in PMs-preserving group （P〈0.05）. The level of serum D- lactic and the rate of organ bacterial translocation were significantly lower in sham operation group （（5.66±0.43）mg/L, （5.6±0.68）%） than those in PMs-preserving group （（12. 62 ±0.89）mg/L, （51. 63±7. 53）%） and PMs-depleting group （（8.96±0.67）mg/L, （40.55±5.67）%） （P〈0.01）, and were significantly lower in PMs-depleting group than those in PMs-preserving group （P〉0.05）. The pancreatic pathologic score was not significant different between PMs- preserving group （7.62±0.9） and PMs depleting group （7.9± 1.2）（P）0.05）. Conclusions Depletion of PMs before establishing model has a protective effect on intestinal barrier function in SAP rats.
出处
《中华实用诊断与治疗杂志》
2014年第6期556-558,共3页
Journal of Chinese Practical Diagnosis and Therapy
