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不同剂量全氟化碳吸入预处理对急性肺损伤实验兔肺组织病理的影响 被引量：4

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摘要 目的探讨不同剂量全氟化碳(PFC)汽化吸入预处理对油酸型急性肺损伤(ALI)实验兔肺组织病理的影响并得到最低有效预处理剂量。方法实验兔24只,采用随机数字表法,将其随机分为4组(n=6),对照组(C组)、PFC1 mL/(kg·h)预处理组(PFC-1)、PFC2 mL/(kg·h)预处理组(PFC-2)、PFC3 mL/(kg·h)预处理组(PFC-3)。动物麻醉后气管插管行机械通气,C组机械通气60 min后建立油酸型ALI模型,3组预处理组分别汽化吸入1、2、3 mL/(kg·h)速率的PFC 60 min,再建立油酸型ALI模型,4组在ALI后继续行机械通气120 min。4组分别于麻醉通气30 min(基础值)、PFC预处理60 min时(C组为机械通气60 min)、ALl造模成功时、ALI后120 min等时点检测氧合指数。实验结束后计算各组实验兔的肺系数,留取右肺作常规病理检查并统计肺叶不同分区病理损伤评分。结果与C组(477±5、472±5、103±5、69±4)比较,3组PFC预处理组[PFC-1(474±11、478±4、122±12、83±5)、PFC-2(472±10、478±4、140±11、88±3)、PFC-3(479±9、480±6、146±15、86±2)]在各时点氧合指数均明显升高(P<0.05),其中PFC-2、PFC-3组氧合指数均高于同时点的PFC-1组(P<0.05);C组的肺系数(8.94±0.51)显著高于各PFC预处理组(P<0.05);各预处理组间的肺系数,PFC-1组(7.93±0.57)相比PFC-2(6.88±0.28)、PFC-3(6.85±0.28)组显著升高(P<0.05),PFC-2与PFC-3比较差异无统计学意义(P>0.05)。各组实验兔肺组织背侧上、中、下叶各区的积分相比同叶同分区的腹侧,均显著升高(P<0.05),并且各组均以下叶背侧的积分值最高。与C组和PFC-1组同侧同分区的积分值均显著高于PFC-2和PFC-3组(P<0.05)。而C组与PFC-1组相比、PFC-2与PFC-3组相比,差异均无统计学意义(P>0.05)。结论经气道2 mL/(kg·h)和3 mL/(kg·h)的PFC吸入两种速率的预处理后,都能改善ALI兔的氧合功能,减少肺组织病理损伤,考虑2 mL/(kg·h)为PFC吸入预处理的最低有效剂量。
作者 李韶辉 郑曦 余革 温晓晖
机构地区 广州医科大学附属第一医院麻醉科 广州医科大学附属肿瘤医院麻醉科 广州医科大学附属第四医院麻醉科
出处 《广东医学》 CAS CSCD 北大核心 2014年第9期1332-1335,共4页 Guangdong Medical Journal
基金 2011年广东省第一批科学事业费计划项目(编号:2011B031800192)
关键词 全氟化碳 预处理 急性肺损伤 病理
分类号 R563 [医药卫生—呼吸系统]
  • 相关文献

参考文献14

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二级参考文献24

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共引文献6

同被引文献27

  • 1陆月明.油酸致急性肺损伤动物模型析评[J].中华急诊医学杂志,2005,14(1):81-83. 被引量：59
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  • 3黄志坚,李琦,王长征,钱桂生,宋鹏.全氟化碳雾化吸入对兔急性肺损伤肺组织结构及抗炎作用的影响[J].中国呼吸与危重监护杂志,2007,6(1):45-48. 被引量：3
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引证文献4

二级引证文献7

广东医学
2014年 第9期

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