摘要
通过测试海南省珠母贝属Pinctada 6个种的45个个体的16S和H3基因序列,并与GenBank数据库中相同物种进行比对,构建系统发育树对其进行分类,同时根据简单、明确的贝壳形态学特征予以最终确认。此分类法不仅可以较准确、快速地进行珠母贝属的分类鉴定,而且分类结果不易受被测试贝类生活环境的影响,不易受分类鉴定人员水平的限制,有利于发现杂交种和隐存种。
A total of 45 individuals from six species of pearl oysters （Pinctada spp. ） were sequenced based on gene 16S and geneH3. NJ trees were constructed by the 45 tested oysters and the individuals from the GenBank database using 16S andH3 sequences. Most of the 45 tested oysters were clustered with the identical species from the GenBank database. With the help of simple and clear shell features, 6 species of Pinctada collected from Hainan Province were identified. This classification method could not only help identify quickly and precisely species of Pinctada, and avoid influences of environmental and human factors, but also could facilitate crossbreeding and discovery of cryptic species of Pinctada.
出处
《热带生物学报》
2014年第1期1-7,共7页
Journal of Tropical Biology
基金
国家重点基础研究发展计划(973计划)(2010CB126405)
海南省青年基金项目(313033)
海南大学科研启动基金资助项目(kyqd1216)
海南大学青年基金资助项目(qnjj1227)
关键词
珠母贝属
16S
H3
形态特征
分类鉴定
Pinctada
16S
H3
shell feature
classification and identification
