摘要
目的考察不同来源葛根药材的质量。方法对湖南省娄底市野生采集(A)、河北安国药材市场(B)、贵州省贵阳市万东药材市场(C)、贵州同济堂制药股份有限公司(D)、四川省成都市荷花池药材市场(E)、北京同仁堂有限责任公司(F)、安徽亳州药材市场(G)、安徽亳州药材市场(H)、云南昭通市药材市场(I)、贵州省大方县野生采集(J)得来的10批葛根药材含水量及水溶性浸出物进行检测,再以高效液相色谱法对葛根素的含量进行测定,色谱条件为:Agilent ZORBAX SB-C18柱(4.6 mm×150.0 mm,5μm),流动相为甲醇-水(25∶75),流速为1 mL/min,检测波长为250 nm,柱温为25℃。结果不同来源的葛根药材中葛根素的含量差异较大,仅B(2.55%)、C(6.39%)、D(4.22%)3种来源的葛根达到《中华人民共和国药典》(2010版)标准(不低于2.4%)。结论不同来源的葛根质量受多方面因素影响,应加强葛根药材生产过程的监管,并对其做进一步的质量控制。
Objective To evaluate the quality of pueraria lobata from different sources. Methods The water contents and water-soluble extracts of pueraria lobata from wild collection in Loudi city of Hunan Province（A）,Anguo Medicine Market of Hebei Province（B）,Guiyang City Wandong Medicine Market of Guizhou Province（C）,Guizhou Tongjitang Pharmaceutical Co., Ltd（D）,Chengdu City Hehuachi Medicine Market of Sichuan Province（E）,Beijing Tongrentang Limited Liability Company（F）, Bozhou Medicine Market of Anhui（G）,Bozhou Medicine Market of Anhui（H）,Zhaotong City Medicine Market of Yunnan（I） and wild collection in Dafang County of Guizhou Province（J） were determined. Meanwhile the content of puerarin in pueraria lobata was detected by high performance liquid chromatography. The liquid chromatography was carried out using Agilent ZORBAX SB-C18（4.6 mm×150.0 mm,5 μm）with the mobile phase of methnol-water（25∶75） at the flow rate of 1 mL/min,the eluent was detected at wavelength of 250 nm,and the temperature was 25 ℃. Results The content of puerarin in pueraria lobata varied in different regions,and only the pueraria lobata from B（2.55%）,C（6.39%）,D（4.22%） reached to the standard of Chinese Pharmacopoeia（2010 ed）（≥2.4%）. Conclusion The quality of pueraria lobata is effected by various factors,it is necessary to strengthen the quality control of manufacture procedures for further quality control of pueraria lobata.
出处
《现代医药卫生》
2014年第10期1454-1456,共3页
Journal of Modern Medicine & Health
关键词
葛根
葛根素
质量评价
Pueraria lobata
Puerarin
Quality evaluation
