摘要
目的探讨丙型肝炎病毒核心抗原(HCV-cAg)检测在丙型肝炎(丙肝)感染窗口期和免疫异常患者中的临床价值。方法 2011年6月至2014年1月分别对丙肝病毒抗体(HCV-Ab)阳性标本153份(Ⅰ组),HCV-Ab阴性血液透析患者、吸毒患者和救助站"三无(无身份证、无工作、无住处)"人员的标本195份(Ⅱ组),HCV-Ab阴性的肿瘤科患者标本207份(Ⅲ组)和HCV-Ab阴性的健康对照标本71份(Ⅳ组),采用酶联免疫吸附试验法检测HCV-cAg;对HCVcAg检测结果阳性标本进一步检测HCV RNA,对HCV-cAg和HCV-Ab均阳性的吸光度值(S/CO)结果进行比较分析。结果在Ⅰ、Ⅱ、Ⅲ、Ⅳ组中HCV-cAg的阳性率分别为29.4%(45/153)、5.6%(11/195)、3.4%(7/207)、1.4%(1/71);HCV-cAg与HCV RNA的符合率达95.3%;随着HCV-cAg水平的下降,HCV-Ab水平呈上升趋势。结论 HCV-cAg检测与HCV RNA符合率高,能防止窗口期和免疫异常丙肝患者的漏诊;HCV-cAg与HCV-Ab互为补充关系,二者联合检测能提高丙肝检出率。
Objective To investigate the clinical value of the hepatitis C virus core antigen （HCV-cAg） in window period and immune abnormalities of the patients with hepatitis C. Methods A total of 153 positive specimens of antibody to hepatitis C virus（HCV-Ab） （groupⅠ）,195 specimens of“three non-personnel”（no ID,no job,no home） in salvation,drug users and hemodial-ysis patients with negative HCV-Ab（groupⅡ）,207 specimens with negative HCV-Ab in Oncology Department（groupⅢ） and 71 specimens of healthy control with negative HCV-Ab（groupⅣ） were adopted from June 2011 to January 2014 by enzyme linked immunosorbent assay to detect the HCV-cAg. The HCV RNA in specimens with positive HCV-cAg were further detected,and the absorbance values（S/CO） of the specimen of positive HCV-cAg and HCV-Ab were compared and analyzed. Results The posi-tive rates of HCV-cAg in groupⅠ,Ⅱ,ⅢandⅣwere 29.4%（45/153）,5.6%（11/195）,3.4%（7/207） and 1.4%（1/71） respec-tively. The coincidence rate of HCV-cAg and HCV RNA reached to 95.3%. With the decline of HCV-cAg level,the level of HCV-Ab reflected upward trend. Conclusion The coincidence rate between HCV-cAg and HCV-RNA is high,HCV-cAg detection can prevent missed diagnosis in the window period and immune abnormalities of HCV patients. HCV-cAg and HCV-Ab show com-plementary relationship,the combined detection can greatly improve the detection rate of hepatitis C.
出处
《现代医药卫生》
2014年第11期1629-1630,1632,共3页
Journal of Modern Medicine & Health
关键词
肝炎
丙型
肝炎病毒属
抗原
病毒
肝炎抗体
酶联免疫吸附测定
Hepatitis C
Hepacivirus
Antigens,viral
Hepatitis antibodies
Enzyme-linked immunosorbent assay
