摘要
[目的]考察不同消毒方式对华中枸骨组织培养的影响。[方法]以华中枸骨的带芽新梢茎段为外植体,分顶芽和侧芽,研究乙醇、升汞的作用时间以及吐温的用量对外植体消毒的影响。[结果]华中枸骨新梢顶芽的最佳消毒方式为:浓度75%乙醇作用50 s,浓度0.1%升汞作用6 min,Tween-80 1滴;侧芽的最佳消毒方式为:浓度75%乙醇作用50 s,浓度0.1%升汞作用7 min。[结论]该方法优化了华中枸骨组织培养的消毒方法,为华中枸骨的进一步开发利用提供依据。
[Objective] To investigate effects of different sterilization pattern on tissue culture of Ilex centrochinensis.[Method] Terminal buds and lateral buds explants of Ilex centrochinensis were sterilized with ethanol,mercuric chloride and Tween to study the effect of different sterilization pattern.[Result] The highest frequency of shoot formation was obtained when terminal buds explants were sterilized with 75％ ethanol 50 seconds,0.1％ mercuric chloride 6 minutes,Tween-80 1 drop,and lateral buds explants were sterilized with 75％ ethanol 50 seconds,0.1％ mercuric chloride 7 minutes.[Conclusion] The method optimized the sterilization pattern of Ilex centrochinensis tissue culture,which will provide reference for further development and utilization of Ilex centrochinensis.
出处
《安徽农业科学》
CAS
2014年第16期5007-5008,共2页
Journal of Anhui Agricultural Sciences
