摘要
该文建立了芪苈强心胶囊中12种有效成分的超高效液相色谱结合质谱（UPLC-MS）的定量方法。采用Phenomenex UPLC Kinetex C18色谱柱（2.1 mm×100 mm,2.6μm）,以乙腈-0.1%甲酸溶液为流动相梯度洗脱,流速0.4 mL·min-1,柱温40℃,进样量5μL,质谱在负离子模式下采用TOF-MS及TOF-MS/MS方法检测。结果表明,12种有效成分毛蕊异黄酮-7-O-葡萄糖苷、异槲皮苷、柚皮芸香苷、橙皮苷、人参皂苷Re、人参皂苷Rg1、杠柳毒苷、人参皂苷Rf、人参皂苷Rb1、黄芪甲苷、人参皂苷Rd、杠柳苷H1在检测的范围内线性良好,R2均大于0.999 0,方法回收率为98.0%~102%,RSD均小于3.9%。该方法简便快速、准确度高,可用于芪苈强心胶囊的质量控制。
In order to establishan UPLC-MS method for determination of twelve active compoundsin Qili Qiangxin capsules including astragaloside,calycosin-7-O-glucoside,ginsenoside Rb1,ginsenoside Re,ginsenoside Rd,ginsenoside Rg1,ginsenoside Rf,periplocin,periplocoside H1,hesperidin,narirutin,isoquercitrin,the chromatographic separations were performedon a Phenomenex UPLC Kinetex C18column（2.1 mm × 100 mm,2.6 μm）with gradient elution of acetonitrile and 0.1% aqueous formic acidat a flow rate of 0.4 mL·min- 1.The temperature was set as 40 ℃ and injection volume was 5 μL.The monitoring of all analytes was achieved under the negative ionization mode with TOF-MS and TOF-MS /MS method.The twelve analytes showed good linearity（ R2 0.999 0）within the test ranges,the average recoveries were 98.0%-102%,respectively,and the RSD were less than 3.9%,respectively.The established method is simple,rapid,and sensitive,and can be used for quality control of Qili Qiangxin capsules.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第10期1822-1825,共4页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973计划)项目(2012CB518606)
国家自然科学基金青年基金项目(81102768)
