摘要
用高表达的转 PEPC基因水稻 HPTER- 0 1作父本 ,与水稻不育系培矮 6 4 S、 2 9130 S、 70 0 1S以及恢复系 512 9、 4 37进行杂交 ,获得 50 5株后代材料 ,鉴定出 4 2株高 PEPC活性和高光合效率的植株。与其母本相比 ,这些材料的 PEPC活性和净光合速率 (Pn)提高 ,CO2 补偿点降低 ,且 Pn 和 PEPC活性呈正相关 ,r=0 .6 6 6 0 * *。从光合日变化上看 ,转育水稻一天中不同时间的光合速率均显著高于其母本 ,特别中午光合光抑制较轻。从光—光合曲线看 ,其量子效率和饱和光合速率亦显著高于其母本。证明通过杂交可以将转 PEPC基因水稻的高光效特性传递到杂种稻的不育系和恢复系中去 ,为常规育种和生物技术相结合的高光效杂种稻的选育提供了依据。
Rice sterile lines Peiai 64S, 29130S, 7001S and restorer lines 5129, 437 as the female parents were crossed with the sixth transgenic rice HPTER 01 containing from maize PEPC gene. Photosynthetic characteristics in the F 1 hybrids and parents were identified. The results showed: (1) 505 hybrid plants were obtained and the PEPC activities among these plants exhibited normal distribution. The percentage of the plants with PEPC activity lying between two parents (100～400 μmol PEP/mgChl.h) was 75.70% and that with high (400~880 μmol PEP/mgChl.h) and low (lower than 100 μmol PEP/mgChl.h) PEPC activity were 8.32% and 4.28%, respectively. (2) 42 hybrid plants were screened and identified with high PEPC activity and high photosynthetic rate. The physiological indexes of the 42 plants were markedly superior to those of their female parents, increased 74.7% in quantum efficiency and 31.3%~110.9% in saturated photosynthetic rate, and decreased 16.2%~17.6% in CO 2 compensation point. The correlation coefficient between PEPC activity and saturated photosynthetic rate (P n) was 0.6660 ** . (3) In compared with their female parents, the photosynthetic rate of the selected plants were obviously high during a fine day, peculiarly exhibiting a slighter photoinhibition of photosynthesis at noon. It is suggested that the PEPC gene in transgenic rice might be incorporated into the rice plant such as the sterile line or restorer line .The precent research may develop a new technical approach for hybrid rice breeding.
出处
《作物学报》
CAS
CSCD
北大核心
2001年第2期137-143,共7页
Acta Agronomica Sinica
基金
国家重点基础研究发展专项经费 !( G1 9980 1 0 1 0 0 )
国家自然科学基金! ( 3 9870 53 3 )
关键词
水稻
PEPC基因
高光效育种
光合生理特性
WT5,5”BZ]Rice
PEPC gene
High photosynthetic efficiency
Photosynthetic characteristic
