摘要
目的观察黄芪虫藤饮对Ⅱ型胶原性关节炎(collagen-induced arthritis,CIA)大鼠的抗炎作用及对大鼠血清血沉(ESR)、类风湿因子(RF)的影响,进一步探讨该药的作用机制。方法将80只SD大鼠随机分为空白组10只,其余建立大鼠CIA模型,将造模成功的大鼠随机分为模型组、甲氨喋呤组、正清风痛宁组、黄芪虫藤饮高、中、低剂量组(下简称高、中、低剂量组)各组10只,分别给药,观察各组大鼠关节炎指数(Arthrits,AI)积分,踝关节病理切片变化,取大鼠血清检测ESR、RF含量。结果 AI积分在给药2、3、4周后,各给药组大鼠与模型组比较,差异有统计学意义(P<0.05或P<0.01),其中各时间点中、低剂量组与高剂量组比较,差异有统计学意义(P<0.05或P<0.01);给药2、3周后高剂量组与正清风痛宁组比较,差异有统计学意义(P<0.05)。各给药组与模型组比较,ESR、RF的含量均降低,差异有统计学意义(P<0.01),中、低剂量组与高剂量组比较,差异有统计学意义(P<0.01);高剂量组RF含量下降比甲氨喋呤组、正清风痛宁组明显,差异有统计学意义(P<0.01)。结论黄芪虫藤饮可降低CIA大鼠关节症状评分,抑制血清ESR、RF升高,对CIA大鼠具有较好的抗炎、免疫调节作用。
Objective To observe the anti-inflammatory effects of Astragalus bug cane drink on typeⅡcollagen-induced arthritis （CIA） rats and the effects on serum erythrocyte sedimentation rate （ESR） and rheumatoid factor （RF）, to further explore the mechanism of action of the drug. Methods SD rats were divided into control group （n=10）, and CIA rats, CIA rats were then randomly divided in-to model group, methotrexate group, Zhengqingfengtongning group, Astragalus bug cane drink high, medium and low dose group （hereinafter referred to as high, medium and low dose group） . Arthritis index （AI）, serum ESR and RF were evaluated; pathological changes of ankle joints were examined. Results AI of treat-ment groups was significantly different from CIA model group in 2, 3, 4 weeks, AI of low-dose group and medium-dose group at each time point was significantly different from high-dose group （P〈0.05 or P〈0.01）. AI of high-dose group was significantly different from Zhengqingfengtongning group after treating 2 to 3 weeks. （P〈0.05）. ESR and RF of treatment group was significantly lower in treat ment groups compared with the model group （ P〈0 . 01 ） , the difference of low-dose group and medium-dose group compared with the high-dose group was statistically significant （P〈0.01）. RF was significantly reduced with high-dose group than MTX group and Zhengqingfengtongning group （P〈0.01）, high-dose group was superior to the methotrexate group and Zhengqingfengtongning groups. Conclusion Astragalus bug cane drink can reduce joint symptoms score, suppress the elevation of serum ESR and RF in type Ⅱ collagen-induced arthritis rats, with anti-inflammatory and im-munomodulatory effects.
出处
《湖南中医药大学学报》
CAS
2014年第5期18-22,共5页
Journal of Hunan University of Chinese Medicine
基金
湖南省科技厅科技计划资助项目(2012SK3140)
关键词
类风湿性关节炎
Ⅱ型胶原性关节炎
黄芪虫藤饮
大鼠
rheumatoid arthritis
type Ⅱ collagen-induced arthritis
Astragalus bug cane drink
SD rats
