期刊文献+

人参皂甙Rb1对感染性休克大鼠重要器官的保护作用 被引量:5

Protective effect of ginsenoside Rb1 on septic shock rats
下载PDF
导出
摘要 目的:探讨人参皂甙Rb1对感染性休克大鼠重要器官的保护作用及其机制。方法:66只SD大鼠随机分为假手术组、模型组、Rb1治疗(0.04 mg/g)组,每组22只。模型组和Rb1治疗组大鼠建立感染性休克动物模型。观察3组大鼠肝、肺组织病理变化,检测血清肿瘤坏死因子-α(TNF-α)含量和心肌组织Toll样受体4(TLR4)mRNA表达的变化。结果:模型组肝细胞肿胀、变性、坏死,中性粒细胞浸润;Rb1治疗组肝细胞浊肿,轻度变性,未见明显坏死灶,肝血窦中中性粒细胞浸润较轻。模型组部分肺泡上皮细胞肿胀,肺泡隔增宽,毛细血管明显扩张淤血,微血栓形成,肺间质充血水肿,弥漫性中性粒细胞浸润,肺泡腔内大量炎性渗出;Rb1治疗组小血管及毛细血管内中性粒细胞浸润较轻,肺间质轻度水肿,肺泡隔增宽不明显,肺泡腔无渗出物。模型组血清TNF-α、心肌组织TLR4mRNA水平高于假手术组,Rb1治疗组两指标低于模型组(P<0.01)。结论:Rb1可保护感染性休克大鼠肝、肺组织,其机制可能与减少TLR4 mRNA表达水平,抑制TNF-α的产生有关。 Aim:To observe the protective effect of ginsenoside Rb 1 on vital organs in rats with septic shock .Meth-ods:The septic shock model was reproduced by CLP .Sixty-six SD rats were randomly divided into three groups ( 22 in each group):sham group, CLP group and ginsenoside Rb1 group(0.04 mg/g).The liver and lung samples were prepared for observation of pathological changes;the expression of Toll like receptor 4(TLR4) mRNA in myocardial tissue was deter-mined by RT-PCR;the serum tumor necrosis factor-α(TNF-α) was measured by ELISA.Results:In CLP group, patho-logical results showed swelling , degeneration , necrosis and neutrophil infiltration in the liver cells , which were milder in ginsenoside Rb1 group.Moreover, in CLP group, there were swelling in alveolar epithelial cells , thickening in alveolar septum, expansion and congestion in capillaries , microthrombosis, congestion and edema in pulmonary interstitial , diffuse infiltration of neutrophils and diminished alveolar space with heavy inflammatory exudation ,which were milder in ginsen-oside Rb1 group.The TLR4 mRNA in myocardial tissue and serum TNF-αin CLP group were significantly higher than those in sham group(P〈0.01).The ginsenoside Rb1 group had markedly lower TLR4 mRNA expression and TNF-αlevel than the CLP group(P 〈0.01).Conclusion: Ginsenoside Rb1 could protect the liver and lung tissue of septic shock rats through down-regulating the expression of TLR 4 mRNA and inhibiting the produce of TNF-α.
出处 《郑州大学学报(医学版)》 CAS 北大核心 2014年第3期319-322,共4页 Journal of Zhengzhou University(Medical Sciences)
基金 河南省科技厅基础与前沿项目102300410163 河南省教育厅科学技术研究重点项目13B320290
关键词 人参皂甙RB1 感染性休克 TOLL样受体4 肿瘤坏死因子-α 大鼠 ginsenoside Rbl septic shock Toll like receptor 4 TNF-α rat
  • 相关文献

参考文献8

二级参考文献49

共引文献145

同被引文献95

引证文献5

二级引证文献28

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部