摘要
目的比较脂多糖(LPS)气管滴注法与雾化吸入法建立的小鼠急性肺损伤(ALI)模型,确立更为有效的小鼠肺损伤建模方法。方法 20只健康雄性C57BL/6小鼠随机分为生理盐水(NS)气管滴注组、NS雾化吸入组、LPS气管滴注组和LPS雾化吸入组。分别采用气管滴注法和雾化吸入法建立肺损伤模型,5 h后进行小鼠肺湿重/干重(W/D)比值测定、支气管肺泡灌洗液(BALF)蛋白含量测定、细胞分类计数及炎性细胞因子检测。结果与对照组相比,LPS气管滴注组和LPS雾化吸入组小鼠肺W/D比值,BALF中总蛋白浓度,TNF-α、IL-6、MCP-1和IL-1β等多项炎性细胞因子以及中性粒细胞数目均显著升高(P均<0.05);NS气管滴注组较NS雾化吸入组炎症背景高;LPS气管滴注组较LPS雾化吸入组各项肺部炎症指标组内差异大。结论雾化吸入方法对于建立小鼠ALI模型更有效。
Objective To compare the mouse models of acute lung injury （ALI） established by intratracheal instillation （IT） and aerosol inhalation （IH） of lipopolysaccharide （LPS）, and to investigate which method was more effective. Methods Twenty male mice were randomly divided into normal saline （NS）-IT group, NS-IH group, LPS-IT group and LPS-IH group. The mice of LPS-IT and LPS-IH groups were exposed to LPS via intratracheal instillation and aerosol inhalation, respectively. The analysis of lung wet/dry weight （W/D） ratio, total protein concentration of bronchoalveolar lavage fluid （BALF）, inflammatory cytokines in BALF and multiple cell countings in BALF were performed 5 h after exposed. Results The lung W/D ratio, total protein concentration of BALF, inlfammatory cytokines such as TNF-α, IL-6, MCP-1 and IL-1βin BALF, and the amount of neutrophils in BALF were signiifcantly increased in the LPS-IT and LPS-IH groups compared with the NS-IT and NS-IH groups （P all 〈 0.05）. Compared with the intratracheal instillation method, the aerosol inhalation method appeared to have lower background inlfammation in the controls and less inter-animal variability. Conclusion The aerosol inhalation is proved to be more effective to establish the mouse model of ALI.
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2014年第2期8-12,共5页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
国家自然科学基金(No.81071587)
关键词
脂多糖
气管滴注法
雾化吸入法
急性肺损伤
Lipopolysaccharide
Intratracheal instillation
Aerosol inhalation
Acute lung injury
