摘要
目的 观察人Po - 5’ -flanking介导的 2 1.5Ku髓鞘碱性蛋白 (myelinbasicprotein ,MBP)微基因 (暂命名为pSVPoMcat)修饰雪旺细胞 (SC)在鼠脊髓内存活及其基因表达。 方法 12 0只大鼠分为 3组 ,A组为pSVPoMcat微基因修饰SC移植组 ;B组为高纯化SC移植组 ;C组为脊髓损伤 (SCI)对照组。伤后各组分别于 2 ,4,8,12周 (每组每次 10只 ) ,取移植区脊髓切片 ,进行S -10 0蛋白、MBP免疫细胞化学染色及地高辛 (DIG)标记的hMBPE2 cDNA探针的原位杂交 (ISH)测定。 结果 伤后 2 ,4,8,12周 ,A组S - 10 0、MBP染色细胞和ISH细胞差异无统计学意义 (P >0 .0 5 ) ,其中ISH阳性细胞可见髓鞘形成。B组的S - 10 0染色细胞逐步递减 ,差异有非常显著性意义 (P <0 .0 1) ,未发现MBP染色细胞以及ISH细胞。C组未发现任何阳性细胞。 结论 pSVPoMcat微基因修饰SC在鼠损伤脊髓内能长期存活并表达外源基因 ,且有助于受伤脊髓的髓鞘形成。
Objective To observe the long term survival and gene expression of genetically modified schwann cell (SC) by pSVPoMcat in spinal cord injuries. Methods The experimental animals were divided randomly into 3 groups, pSVPoMcat modifying SC implantation group (Group A), SC implantation group (Group B) and control group (Group C). After 3 months, the sections of grafted spinal cord were checked by immunocytochemistry and in situ hybridization in 3 groups. Results There was no significant difference among staining positive cells, S 100 MBP and positive ISH cells in Group A. The myelin of positive ISH cells rounded new regeneration axons. A few positive cells of MBP were seen in Group B, no positive cells in Group C. Conclusions SC genetically modified by pSVPoMcat has a long term survival in spinal cord injuries and helps to form myelin of spinal cord injury.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2001年第2期86-88,共3页
Chinese Journal of Trauma
