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amiRNA技术及其在食用作物遗传改良中的应用 被引量:1

amiRNA technology and its applications in alimentary crop genetic improvement
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摘要 内源miRNAs(microRNAs)是负调控真核生物基因表达的关键因子。amiRNAs(artificial microRNAs)是模拟内源miRNA的生成途径人工合成的miRNA,它能高效抑制靶基因的表达。运用amiRNA技术调控基因表达在调控精确性、效率、遗传稳定性及生物安全性等多方面具有显著优势。本文就amiRNA技术的原理、优势以及该技术在食用作物品质改良及营养改善、雄性不育系培育、抗病毒和抗逆作物培育中的应用进行了综述。 Endogenous miRNAs(microRNAs) are key factors which negatively regulate gene expression in eukaryotes, amiRNAs (artificial miRNAs) are the artificial miRNAs which are synthesized by simulating the synthesis pathway of endogenous miRNAs, amiRNAs can effectively inhibit the expression of target genes. Application of amiRNA technology in the regulation of gene expression has significant advantages in accuracy, efficiency, genetic stability and biology security. In this review,the principle and advantages of amiRNA technique, and the applications in alimentary crop quality improvement ,nutrition improvement ,male sterile lines breeding, anti-viral and anti-adversity crop breeding were summarized.
出处 《食品工业科技》 CAS CSCD 北大核心 2014年第12期388-392,397,共6页 Science and Technology of Food Industry
基金 国家自然科学基金资助(31060044 31260070)
关键词 amiRNA 食用作物 基因沉默 遗传改良 应用 amiRNA alimentary crop gene silencing genetic improvement application
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  • 1Kruszka K,Pieczynski M,Windels D,et al. Role of microRNAs and other sRNAs of plants in their changing environments [J]. Journal of Plant Physiology,2012,169(16) : 1664-1672.
  • 2Lee Y,Kim M,Han J,et al. MicroRNA genes are transcribed by RNA polymerase II[J]. The EMBO Journal,2004,23(20): 4051-4060.
  • 3Kurihara Y,Watanabe Y. Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004,101(34) : 12753-12758.
  • 4Hofmann N R. MicroRNA evolution in the genus Arabidopsis [J]. The Plant Cell, 2010,22(4 ) : 994.
  • 5Hammond S M, Bernstein E, Beach D, et al. An RNA- directed nuclease mediates post-transcriptional gene silencing in Drosophila cells[J]. Nature, 2000,404: 293-296.
  • 6Park M Y,Wu G,Gonzalez-sulser A,et al. Nuclear processing and export of microRNAs in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005,102( 10):3691-3696.
  • 7Perez-quintero A L,Lopez C. Artificial microRNAs and their applications in plant molecular biology[J]. Agronomia Colombiana, 2010,28( 3 ) : 373-381.
  • 8Schwab R,Ossowski S,Riester M,et al. Highly specific gene silencing by artificial microRNAs in A rabidopsis[J]. The Plant Cell,2006,18(5): 1121-1133.
  • 9叶梅霞,崔东清,李昊,王静澄,刘军梅,张志毅,安新民.amiRNA分子设计及其离体合成策略[J].中国生物工程杂志,2010,30(9):92-97. 被引量:2
  • 10Schwarb R,Ossowski S,Warthmann N,et al. Directed gene silencing with artificial microRNAs[J]. Methods in Molecular Biology, 2010,592 : 71-88.

二级参考文献143

  • 1Zhao T, Wang W, Bai X, et al. Gene silencing by artificial microRNAs in Chlamydomonas. Plant J, 2009, 58 ( 1 ) : 157- 164.
  • 2Liu C, Zhang L, Sun J, et al. The artificial microRNA mediates GUS-GF Pgene silencing using ath-miR169d precursor as backbone. Life Sci J, 2009, 6 (2) : 1-7.
  • 3Hu T, Fu Q, Chen P, et al. Construction of an artificial microRNA expression vector for simultaneous inhibition of multiple genes in mammalian cells. Int J Mol Sci, 2009, 10 (5) : 2158-2168.
  • 4Schwab R, Palatnik J F, Riester M, et al. Specific effects of microRNAs on the plant transcriptome. Dev Cell, 2005, 8 (4) : 517-527.
  • 5Ossowski S, Schwab R, Weigel D. Gene silencing in plants using artificial microRNAs and other small RNAs. Plant J, 2008, 53 (4) : 674-690.
  • 6Schwab R. Cloning of artificial microRNAs, 2005, (http:// wind3, weigelworld, org/cgi-bin/webapp, cgi? page = Downloads ; project = stdwmd).
  • 7Schwab R, Ossowski S, Riester M, et al. Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell, 2006, 18 (5): 1121-1133.
  • 8Geu-Flores F, Nour-Eldin H H, Nielsen M T, et al. USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products. Nucleic Acids Res, 2007, 35 (7) : e55.
  • 9Nour-Eldin H H, Hansen B G, Norhohn M H, et al. Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments. Nucleic Acids Res, 2006, 34 (18) : e122.
  • 10McIntyre G J, Fanning G C. Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnol, 2006, 6:1.

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  • 1Bendahmane M,Chen I,Asurmendi S,et al. Coat protein- mediated resistance to TMV infection of Nicotiana taba- cttm involves multiple modes of interference by coat pro- tein [ J ]. Virology ,2007,366 ( 1 ) : 107-116.
  • 2Smith N A,Singh S P,Wang M B,et al. Total silencing by intron-spliced hairpin RNAs [ J ]. Nature, 2000, 407 : 319-320.
  • 3Fahim M, Larkin P J. Designing effective amiRNA and multimeric amiRNA against plant viruses [ J]. Methods in Molecular Biology ,2013,942:357-377.
  • 4Naqvi A R, Sarwat M, Hasan S,et al. Biogenesis, functions and fate of plant microRNAs [ J ]. Journal of Cellular Phy- siology, 2012,227 ( 9 ) : 3163-3168.
  • 5Yu B,Wang H. Translational inhibition by microRNAs in plants [ J ]. Progress in Molecular and Subcellular Biology, 2010,50:41-57.
  • 6Niu Q W, Lin S S, Reyes J L, et al. Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers vi- rus resistance[ J ]. Nature Biotechnology, 2006,24 ( 11 ) : 1420-1428.
  • 7Tiwari M, Sharma D, Trivedi P K. Artificial microRNA mediated gene silencing in plants: Progress and perspec- tives[ J]. Plant Molecular Biology ,2014,86 (1/2) :1-18.
  • 8Qu J, Ye J, Fang R. Artificial microRNA-mediated virus resistance in plants [ J ]. Journal of Virology, 2007,81 ( 12 ) :6690-6699.
  • 9Ai T,Zhang L, Gao Z,et al. Highly efficient virus resis- tance mediated by artificial microRNAs that target the suppressor of PVX and PVY in plants [ J ]. Plant Biolo- gy,2011,13(2) :304-316.
  • 10Kung Y J,Lin S S,Huang Y L,et al. Multiple artificial microRNAs targeting conserved motifs of the replicase gene confer robust transgenic resistance to negative-sense single-stranded RNA plant virus [ J ]. Molecular Plant Pathology, 2012,13 ( 3 ) : 303 -317.

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