摘要
目的探讨CD38对滤泡性淋巴瘤(FL)和反应性滤泡增生(RFH)的鉴别诊断价值。方法对25例RFH和42例FL病例进行形态学及免疫组化对照分析。结果 25例RFH病例均显示CD38在反应性生发中心呈典型双相表达模式:生发中心弥漫弱表达CD38,此外还有少量CD38强阳性细胞,后者主要位于明暗区交界处与明区。CD38在FL中有2种表达模式:模式1(32例)显示肿瘤性滤泡中的瘤细胞弥漫一致的弱-中等阳性,其中30例完全没有强阳性细胞,2例仅在个别滤泡有很少强阳性细胞;模式2(10例)显示肿瘤性滤泡CD38完全阴性。共同特点是无论瘤细胞是否表达CD38,肿瘤性滤泡中缺乏强阳性细胞,或仅在个别滤泡见到很少强阳性细胞。结论 CD38在FL和RFH中有截然不同的表达模式,可为二者的鉴别提供新的依据。
Objective To explore the significance of expression of CD38 in differential diagnosis of follicular lymphoma （FL） from reactive follicular hyperplasia （RFH） in immunodetection of paraffin - embedded tissue sections. Methods Twenty - five specimens of RFH and forty - two specimens of FL were examined by light microscopy and immunohistoehemieal staining. Results In RFH cases, the immunoreaetivity of CD38 was observed in centrocytes and centroblasts throughout the germinal center （GC） with weak to moderate uniform staining intensity, and a subset of strongly positive cells predominantly located in the light zone, indicating a typical dual expression pattern. CD38 staining revealed three patterns in FL. In pattern I （32 cases）, tumor cells maintained CD38 expression within the neoplastic follicles, which exhibited weak to moderate uniform staining intensity. In 30 of these cases, the neoplastic follicles were entirely devoid of CD38 strongly positive cells, whereas only rare, scattered strongly positive cells were observed in a minority of neoplastic follicles in the remaining two cases. In pattern II （ 10 eases）, tumor cells in neoplastic follicles exhibited loss of CD38 expression accompanied with the disappearance of strongly positive plasma cell predursors. Conclusion In comparison with the reactive GCs, the almost entire lack of cells with strong expression of CD38 in follicular structures was the most striking alteration. The results presented here demonstrate that easy method for differentiating FL from RFH staining for CD38 in paraffin - embedded sections is a simple and
出处
《临床和实验医学杂志》
2014年第12期958-960,共3页
Journal of Clinical and Experimental Medicine
基金
国家自然科学基金资助项目(81272633)
