摘要
为了用响应曲面法优化微波辅助提取苦荞籽总黄酮的工艺,试验选用微波辅助法提取苦荞籽总黄酮,并选取乙醇浓度、料液比、提取时间、微波预处理时间4个因素进行单因素试验,在此基础上以4个主要因素(乙醇浓度、料液比、提取时间、微波时间)为自变量,提取率为响应值,采用Box-Behnken试验设计和响应面分析法,建立数学模型。结果表明:确定苦荞仔黄酮提取的最佳工艺条件为乙醇浓度58.25%,料液比27.97∶1,提取时间2.38 h,微波时间107.48 s;在此条件下,苦荞仔总黄酮的得率5.05%。说明由响应曲面法得到的优化工艺参数比较准确,具有实用意义。
To optimize the microwave assisted extraction process of flavonoids from tartary buckwheat seeds using response surface methodology （RSM）. The microwave - assisted extraction was used to extract total flavonoids （TF） from tartary buckwheat seeds, and the following four fac- tors were selected to be used for a single factor test, including the ethanol concentration, liquid - material ratio, extraction time and microwave pretreatment time ; the Box - Behnken experimental design and RSM were used to build a mathematical model with the extraction rate as the re- sponse value based on the four factors as independent variables, including the ethanol concentration, liquid - material ratio, extraction time and microwave time. The results showed that the optimal conditions for the extraction of flavonoids from tartary buckwheat seeds were determined as follows ： the ethanol concentration of 58.25 %, the liquid - material ratio of 27.97：1, the extraction time of 2.38 h, and the microwave time of 107.48 s. Under these conditions, the obtained extraction yield of total flavonoids from tartary buckwheat seeds was 5.05%. The results indicate the technological parameters optimized by RSM are more accurate,which have practical significance for the extraction of total flavonoids from tar- tary buckwheat seeds.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2014年第6期34-38,共5页
Heilongjiang Animal Science And veterinary Medicine
关键词
苦荞籽
黄酮
微波辅助提取
响应曲面法
Box—Behnken试验设计
tartary buckwheat seeds
flavonoids
microwave assisted extraction
response surface methodology
Box- Behnken experimental de-sign
