摘要
采用双抗体夹心酶联免疫吸附试验(ELISA)测定40例儿童支气管哮喘发作期、20例缓解期血清白细胞介素1(IL-1)、白细胞介素6(IL-6)、白细胞介素8(IL-8)水平。并设20例健康儿童作正常对照。结果:发作期IL-1(11.90pg/ml±2.84pg/ml)、IL-6(0.75ng/ml±0.30ng/ml)、IL-8(0.08ng/ml±0.05ng/ml)水平明显高于缓解期(8.41pg/ml±0.05pg/ml、0.49ng/ml±0.08ng/ml、0.02ng/ml±0.01ng/ml)及对照组(8.40pg/ml±0.32pg/ml、0.40ng/ml±0.09ng/ml、0.02ng/ml±0.02ng/ml),经统计学处理有显著差异(P<0.01)。且血清IL-1水平与IL-6、IL-8比较呈正相关(γ=0.753,γ=0.795,P<0.01)。提示IL-1与IL-6、IL-8一样参与了哮喘的病理过程,哮喘缓解期仍存在气道炎症反应。细胞因子网络失衡可能是哮喘发病的分子生物学基础。可将血清IL-1、IL-6、IL-8水平升高作为支气管哮喘发作的指标之一。
Serum IL-1,IL-6 and IL-8 were determined by subjects-using enzyme linked immunosorbent assay (ELISA technique) in 40 children with bronchial asthma consisting of 20 cases in the acute stage and 20 cases in the remission stage, and another 20 health children as normal control,respectively. The results showed that the levels of serum IL-1(11. 9pg/ml± 2. 84pg/ml),IL-6(0. 75ng/ml±0. 3ng/ml)and IL-8(0. 08ng/ml±0. 05ng/ml)in the acute stage were significantly higher than those in the remission stage(8. 41pg/ml±0. 05pg/ml(IL-1) ,0. 49ng/ml±0. 08ng/ml(IL-6)and 0. 02ng/ml±0. 01ng/ml(IL-8)) and in the normal children (8. 4pg/ml±0. 32pg/ml(IL-1),0. 4ng/ml±0. 09ng/ml(IL-6)and 0. 02ng/ml±0. 02ng/ml(IL-8)),respectively. Further more,there were a significant positive correlation between IL-1 and IL-6 or IL-8(.P<0. 01). It is concluded that IL-1 may involve in the pathologic process of asthma like IL-6 and IL-8,and an inflammatory reaction may still exist in the airway during the remission stage of asthma. The imbalances of cytokine network may probably be important reason as a molecular basis in the asthma pathogenesis. It is suggested that it can be valuable to predict an acute attack of asthma when the levels of these cytokines increase.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2001年第1期7-8,31,共3页
Journal of Clinical Pediatrics
基金
卫生部科学研究基金
