摘要
目的:为优化乳清蛋白肽-钙的螯合工艺,在单因素试验的基础上应用响应面法对乳清蛋白肽与氯化钙的螯合工艺进行优化,确定最优水平,得到乳清蛋白肽-钙螯合物,以期为人们提供1种新型的保健食品。方法:利用复合蛋白酶和复合风味蛋白酶酶解乳清蛋白,将所得乳清蛋白肽与钙离子进行螯合反应,优化工艺制备螯合产物,并利用红外光谱法和荧光光谱法对乳清蛋白肽-钙螯合物进行表征。结果:根据响应面分析,得到优化的乳清蛋白螯合钙的制备条件为:以液体钙的形式添加,乳清蛋白肽与氯化钙质量比24∶1,乳清蛋白肽质量浓度35 mg/mL,反应时间20 min,反应温度30℃,pH 7.0。采用红外光谱法和荧光光谱法对螯合物进行表征,表明所得物质为乳清蛋白肽与钙的螯合物。结论:经酶水解得到的乳清多肽能够与钙离子螯合。优化得到最佳螯合工艺,为乳清蛋白金属螯合产品的生产和开发提供了新的思路。
Objective： To optimum the chelation technology of whey protein peptides with calcium, based on single factor tests, the response surface method was used to investigate optimized technological conditions. Finally, the optimum level was established and whey protein peptide-calcium chelation was obtained, which can explore the new function of whey proteins and metal ion chelate compound and provide a new type of health food. Methods： Whey protein was hydrolysed by protemax and flavourzyme, then process optimization for chelating reaction between whey protein peptides and calcium was conducted using response surface method. After this, the characters of chelate compounds were detected by infrared spectrometry and fluorescent spectrometry. Results： The optimum process parameters for the whey protein peptides-calcium chelation： the calcium was added in the form of liquid, whey protein peptide and CaCI2 ratio of 24：1 （m/ m）, whey protein peptide concentration 3.5%, reaction time 20min, reaction temperature 30℃, pH 7.0; the whey pro- tein chelated calcium was characterized by infrared spectrometry and fluorescent spectrometry, the results showed that the whey protein peptides had successfully chelated onto calcium. Conclusion： The whey protein peptides obtained after enzymatic hydrolysis have successfully chelated onto calcium in the CaCl2; The chelates provide new opportunities for whey protein composite products＇ production and development.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2014年第4期64-71,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
福建省自然科学基金项目(2013J01132)
福建省新世纪优秀人才支持计划项目(JA10012)
关键词
乳清蛋白肽
螯合
多肽-钙螯合物
响应面法
whey protein peptide
chelation
polypeptide-Ca2＋
response surface method
