摘要
目的建立一种离子对高效液相色谱方法，简便、快速检测减肥类保健食品中左旋肉碱含量。方法采用ODS柱进行分离，以40mmol／L磷酸二氢钾缓冲溶液-乙腈（95：5）（含0．1％己烷磺酸钠，用0．1mol／L盐酸调节pH值至2．6）为流动相，流速：0．8mL/min，检测波长：220nm定量管进样20μL。结果在20-200μg／mL的浓度范围内，峰面积与进样浓度呈良好的线性关系，相关系数r=0．9996，加样回收率为98．5％，峰面积和保留时间日内精密度RSD分别为1．7％、0．9％；日间精密度RSD分别为3．8％、1．2％。结论方法简便、快速，可用于含左旋肉碱保健食品的含量检测。
Objective To establish a convenient and rapid method to determine L-carnitine in slimming health products by ion-pair high performance liquid chromatography （HPLC）. Methods An ODS column was used for separation, with 40 mmol potassium phosphate monobasic solution-acetonitrile （95：5 ） （containing 0.1% sodium hexanesulfonate, adjusted to pH2.6 with 0.1 mol/L hydrochloride acid solution） as the mobile phase. The flow rate was 0. 8 mL/min, and detect wavelength was 220 nm. Results A good linear calibration curve was obtained in the range of 20-200μg/mL, with a correlation coefficient of 0.999 6. The spiked recovery was 98.5%. The intra-day RSDs of peak area and retention time were 1.7% and 0.9%, and inter-day RSDs were 3.8% and 1.2% respectively. Conclusion The method is convenient and rapid for the analysis of L-carnitine in slimming health products.
出处
《今日药学》
CAS
2014年第5期337-339,共3页
Pharmacy Today
关键词
左旋肉碱
保健食品
离子对色谱
L-carnitine
health food
ion-pair high performance liquid chromatography
