摘要
目的合成甲基对氧磷 (M16 0 0 )人工抗原。方法用醋酸 -锌粉盐酸还原对氧磷 ,制备氨基甲基对氧磷 ,重氮化法使氨基甲基对氧磷与牛血清白蛋白 (BSA)及中国鲎血蓝蛋白 (TTH )偶合 ,合成人工抗原 M16 0 0 - BSA、M16 0 0 - TTH。结果M16 0 0 - BSA免疫新西兰兔 10周后 ,双向琼脂扩散试验和间接 EL ISA检测 ,证明得到了高价且具有较好特异性的多抗血清。结论成功地合成了甲基对氧磷人工抗原 。
ObjectiveTo synthesize the artificial antigen methylparaoxon (M1600) MethodsMethylparaoxon was reduced into amino methylparaoxon by using acetic acid zinc power hydrochloric acid Artificial antigens M1600 BSA, M1600 TTH were synthesized by conjugating amino methylparaoxon to bovine serum albumin (BSA)and tachypleus tridentatus hemocyanin (TTH)directly after diazotization Results Rabbits had been immunized with M1600 BSA for 10 weeks, and the high titer and high specificity antiserum from those rabbits was testified by doubled agar gel diffusion and indirect ELISA.ConclusionAn artificial antigen was obtained successfully and this made it possible to establish the immunoassay of M1600
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第2期144-145,154,共3页
Immunological Journal
