摘要
目的:探讨氨氯吡咪(Amiloride)通过抑制血管内皮细胞生长因子mRNA(VEGF mRNA)表达,观察VEGF mRNA与K562细胞凋亡是否有关。方法:将不同浓度Amiloride与K562细胞共同孵育,采用Annexin VFITC/PI双标记流式细胞术检测细胞凋亡。同时采用RT-PCR技术观察Amiloride对K562细胞VEGF mRNA表达的影响,免疫组化方法观察Amiloride对K562细胞VEGF蛋白表达的影响。应用MTT法观测Amiloride对K562细胞增殖的影响。结果:不同浓度Amiloride作用K562细胞24h后,K562细胞的增殖受到抑制,VEGF mRNA表达强度的相对系数及VEGF蛋白呈浓度依赖性下降,并且可以观察到随着VEGF mRNA表达强度的相对系数的降低,K562细胞凋亡率增加,二者之间显著负相关(P<0.05)。结论:氨氯吡咪可能通过抑制VEGF mRNA表达促进K562细胞凋亡。
Objective： To explore the relationship between vascular endothelial growth factor （VEGF） mRNA and cell apoptosis, and the effect of amiloride on the apoptosis of K562 cells. Methods： K562 cells were incubated with different concentrations of amiloride, and then cell apoptosis was assayed by AnnexinV-FITC/PI double staining. Meanwhile, the expression of VEGF mRNA in K562 cells was measured by RTPCR and the expression of VEGF protein were measured by immunohistochemistry assay. MTT test was used to examine the influence of amiloride on the pro- liferation of K562 cells. Results： After K562 cells were treated with different concentrations of amiloride for 24 h, proliferation of K562 cells were inhibited, the levels of VEGF mRNA expres- sion and VEGF protein in the K562 cells decreased remarkably in a concentration-dependent man- ner. With the decreasing level of VEGF mRNA expression, cell apoptosis rates increased gradu- ally （P〈0.05）. Conclusion： Amiloride could promote apoptosis of K562 cells by inhibiting the expression of VEGF mRNA.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2014年第3期362-365,共4页
Medical Journal of Wuhan University
